| Stimulation by bone sialoprotein of calcification in osteoblast-like MC3T3-E1 cells. | |
| | |
MedLine Citation:
|
PMID: 7621349 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bone sialoprotein (BSP) containing an Arg-Gly-Asp cell-binding sequence was purified from bovine bone 4 M guanidine-HCl extract after HCl demineralization by a series of chromatographic procedures. When this protein was coated on culture dishes in the presence of type I collagen, it increased both DNA content and alkaline phosphatase (ALP) activity in osteoblast-like MC3T3-E1 cells, and stimulated calcification in the cells, whereas fibronectin, another cell-binding protein, showed a marked increase in the DNA content but had little effect on the ALP activity. These findings suggest that BSP is mitogenic for preosteoblasts and differentiating the cells into osteoblasts, thereby stimulating bone calcification. |
| | |
Authors:
|
H Y Zhou; H Takita; R Fujisawa; M Mizuno; Y Kuboki |
Related Documents
:
|
3513169 - Inhibition of tumor-cell-mediated extracellular matrix destruction by a fibroblast prot... 19568599 - Phenotypic characterization of ligamentum flavum cells from patients with ossification ... 10529909 - Effect of impact load on articular cartilage: cell metabolism and viability, and matrix... 20558219 - Cellular phenotype switching and microvesicles. 10910039 - Front-cell-specific expression of membrane-type 1 matrix metalloproteinase and gelatina... 9724899 - Small intestinal submucosa: a substrate for in vitro cell growth. 21046399 - Cobalt immobilization by manganese oxidizing bacteria from the indian ridge system. 22218929 - Migration of intervertebral disc cells into dense collagen scaffolds intended for funct... 6467209 - Comparative studies of heat sensitivity of several rat hepatoma cell lines and hepatocy... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Calcified tissue international Volume: 56 ISSN: 0171-967X ISO Abbreviation: Calcif. Tissue Int. Publication Date: 1995 May |
Date Detail:
|
Created Date: 1995-08-30 Completed Date: 1995-08-30 Revised Date: 2010-02-04 |
Medline Journal Info:
|
Nlm Unique ID: 7905481 Medline TA: Calcif Tissue Int Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 403-7 Citation Subset: IM; S |
Affiliation:
|
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3T3 Cells Alkaline Phosphatase / metabolism Amino Acid Sequence Animals Binding Sites Calcification, Physiologic / drug effects* DNA / metabolism Mice Molecular Sequence Data Osteoblasts / cytology, drug effects, physiology* Sialoglycoproteins / isolation & purification, pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Sialoglycoproteins; 0/integrin-binding sialoprotein; 9007-49-2/DNA; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: EDTA-insoluble, calcium-binding proteoglycan in bovine bone.
Next Document: Appendicular cortical bone loss after age 65: sex-dependent event?