| Stimulation of astrocyte-enriched culture with C2 ceramide increases proenkephalin mRNA: involvement of cAMP-response element binding protein and mitogen activated protein kinases. | |
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MedLine Citation:
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PMID: 11382404 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In rat astrocyte-enriched culture, C2 ceramide dose- and time-dependently increased proenkephalin (proENK) mRNA; the significant increase began at 6 h after 30 microM C2 ceramide treatment (about 13-fold) and at 12 h after treatment (about 21-fold). In addition, C2 ceramide also increased AP-1 proteins, such as Fra-1, c-Jun, JunB and JunD, and phosphorylation of CREB. The blocking of protein synthesis by cycloheximide (CHX) evokes a further increase of C2 ceramide-induced proENK mRNA and phospho-CREB level, while C2 ceramide-induced increases of AP-1 protein levels were reduced by CHX. The C2 ceramide-induced proENK mRNA expression was not changed significantly by the pretreatment with H89 (a PKA inhibitor), KN62 (a calcium/calmodulin-dependent protein kinase II inhibitor), and PD98059 (an ERK pathway inhibitor). However, calphostin C (a PKC inhibitor) and or SB203580 (a p38 inhibitor) partially but significantly reduced C2 ceramide-induced proENK mRNA expression as well as phospho-CREB level. These results suggest that, in the rat astrocyte-enriched culture, C2 ceramide increases proENK mRNA expression via phosphorylation of CREB rather than the increases of AP-1 protein levels. Additionally, the activations of PKC and p38, but not PKA, calcium/calmodulin-dependent protein kinase II, and ERK, by C2 ceramide play important regulatory roles in C2 ceramide-induced proENK mRNA expression via activating the CREB. |
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Authors:
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J S Won; M R Choi; H W Suh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research Volume: 903 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2001 Jun |
Date Detail:
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Created Date: 2001-05-30 Completed Date: 2001-08-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 207-15 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, College of Medicine, Hallym University, 1 Okchun-Dong, Chunchon, Kangwon-Do 200-702, South Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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analogs & derivatives,
pharmacology Animals Astrocytes / cytology, enzymology* Cells, Cultured Cyclic AMP Response Element-Binding Protein / metabolism* Cycloheximide / pharmacology Enkephalins / genetics* Enzyme Inhibitors / pharmacology Flavonoids / pharmacology Gene Expression / drug effects Imidazoles / pharmacology Isoquinolines / pharmacology Mitogen-Activated Protein Kinases / metabolism* Naphthalenes / pharmacology Phosphorylation Protein Precursors / genetics* Protein Synthesis Inhibitors / pharmacology Pyridines / pharmacology RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Sphingosine / analogs & derivatives, pharmacology* Stimulation, Chemical Sulfonamides* Transcription Factor AP-1 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cyclic AMP Response Element-Binding Protein; 0/Enkephalins; 0/Enzyme Inhibitors; 0/Flavonoids; 0/Imidazoles; 0/Isoquinolines; 0/N-acetylsphingosine; 0/Naphthalenes; 0/PD 98059; 0/Protein Precursors; 0/Protein Synthesis Inhibitors; 0/Pyridines; 0/RNA, Messenger; 0/SB 203580; 0/Sulfonamides; 0/Transcription Factor AP-1; 0/proenkephalin; 121263-19-2/calphostin C; 123-78-4/Sphingosine; 127191-97-3/KN 62; 127243-85-0/H 89; 66-81-9/Cycloheximide; 84477-87-2/1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; EC 2.7.11.24/Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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