| Stimulation of TLR7 prior to polymicrobial sepsis improves the immune control of the inflammatory response in adult mice. | |
| | |
MedLine Citation:
|
PMID: 20953969 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
OBJECTIVE: The role of Toll-like receptor 7 (TLR7), so far regarded as a receptor for viral RNA, was evaluated in a murine sepsis model. MATERIAL: We used the colon ascendens stent peritonitis model (CASP) in female C57B/6 mice. R-848 (1.5 μg/g body weight) was injected intravenously prior to sepsis induction. METHODS: We determined levels of cytokines by CBA detection kit. Different cell populations were isolated from the spleen by magnetic cell separation and the expression of TLR7 was visualized by immunofluorescence staining. Bacterial load of organs was quantified by incubating suspensions on agar in colony forming units. RESULTS: R-848 application per se led to elevated cytokine levels in serum, spleen and peritoneal cavity. Expression of TLR7 on splenocytes was upregulated following CASP. Bacterial clearance in polymicrobial sepsis was significantly increased in spleen and peritoneum of mice pre-treated with the TLR7-agonist. Cytokine release was regulated in the peritoneum and spleen. Furthermore, apoptosis in thymus and spleen during polymicrobial sepsis was significantly decreased following TLR7 agonist application. CONCLUSIONS: TLR7 seems to be essential for pathogen defence not only in viral but also in bacterial infections. Pharmacological stimulation of this receptor prior to induction of sepsis improves the host's capacity to cope with pathogens. |
| | |
Authors:
|
P Koerner; T Traeger; H Mehmcke; K Cziupka; W Kessler; A Busemann; S Diedrich; G Hartmann; C-D Heidecke; S Maier |
Related Documents
:
|
12004239 - Endothelium: interface between coagulation and inflammation. 16237649 - Prevention of lymphocyte apoptosis--a potential treatment of sepsis? 8924969 - Applied molecular biology of sepsis. 12488489 - Hmgb1 as a dna-binding cytokine. 16513409 - High-mobility group box 1 (hmgb1) protein: friend and foe. 10785649 - Inflammation-coagulation network: are serine protease receptors the knot? 22289049 - Melatonin enhances thapsigargin-induced apoptosis through reactive oxygen species-media... 10463949 - Death deflected: il-15 inhibits tnf-alpha-mediated apoptosis in fibroblasts by traf2 re... 11100839 - Selective light-induced modulation of bcl-xl and bax expressions in indocyanine green-l... |
Publication Detail:
|
Type: Journal Article Date: 2010-10-17 |
Journal Detail:
|
Title: Inflammation research : official journal of the European Histamine Research Society ... [et al.] Volume: 60 ISSN: 1420-908X ISO Abbreviation: Inflamm. Res. Publication Date: 2011 Mar |
Date Detail:
|
Created Date: 2011-02-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9508160 Medline TA: Inflamm Res Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 271-9 Citation Subset: IM |
Affiliation:
|
Department of Surgery, General Surgery, Visceral, Thoracical and Vascular Surgery, Ernst Moritz Arndt Universität, Friedrich-Loeffler-Str 23b, 17475, Greifswald, Germany, koernerp@uni-greifswald.de. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Proinflammatory cytokines in the cerebrospinal fluid of patients with lumbar radiculopathy.
Next Document: Exogenous procalcitonin evokes a pro-inflammatory cytokine response.