| Stimulation of the T-cell antigen receptor-CD3 complex signaling pathway by the tyrosine phosphatase inhibitor pervanadate is mediated by inhibition of CD45: evidence for two interconnected Lck/Fyn- or zap-70-dependent signaling pathways. | |
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MedLine Citation:
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PMID: 8734787 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The tyrosine phosphatase specific inhibitor pervanadate is a potent activator of T lymphocytes through induction of tyrosine phosphorylation and downstream events of the activation cascade. Using CD45- or CD3-negative variants of the Jurkat leukemic T-cell line we show that the different biochemical events induced by pervanadate appeared to be dependent on the presence at the cell surface of either CD45 or CD3. CD45-dependent events such as tyrosine phosphorylation of Shc, activation of nuclear factor-kappa B (NF-kappa B), activator protein-1 (AP-1), transcription factors, and stimulation of interleukin-2 (IL-2) promoter and of CD69 and CD25 surface expression paralleled activation of the tyrosine kinases lck and fyn. By contrast, stimulation of calcium influx, a CD3-dependent event, paralleled zap-70 activation. The data demonstrate that the T-cell antigen receptor-CD3 (TcR-CD3) complex is functionally linked to two different protein tyrosine kinase (PTK) modules with separate specific functions and that CD45 may be an important regulator of this coupling. |
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Authors:
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V Imbert; D Farahifar; P Auberger; D Mary; B Rossi; J F Peyron |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of inflammation Volume: 46 ISSN: 1078-7852 ISO Abbreviation: J. Inflamm. Publication Date: 1996 |
Date Detail:
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Created Date: 1996-10-10 Completed Date: 1996-10-10 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9511967 Medline TA: J Inflamm Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 65-77 Citation Subset: IM |
Affiliation:
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INSERM Unité 364, Faculté de Médecine Pasteur, Nice, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD3
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immunology* Antigens, CD45 / immunology* Base Sequence Binding Sites DNA / chemistry, metabolism Enzyme Inhibitors / pharmacology Humans Interleukin-2 / genetics Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Molecular Sequence Data Promoter Regions, Genetic Protein Tyrosine Phosphatases / antagonists & inhibitors* Protein-Tyrosine Kinases / metabolism Receptors, Antigen, T-Cell / immunology* Signal Transduction / drug effects* T-Lymphocytes / immunology Transcription Factors / metabolism Tumor Cells, Cultured Vanadates / pharmacology* ZAP-70 Protein-Tyrosine Kinase src-Family Kinases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD3; 0/Enzyme Inhibitors; 0/Interleukin-2; 0/Receptors, Antigen, T-Cell; 0/Transcription Factors; 0/Vanadates; 0/pervanadate; 9007-49-2/DNA; EC 2.7.1.112/ZAP70 protein, human; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/Lymphocyte Specific Protein Tyrosine Kinase p56(lck); EC 2.7.10.2/ZAP-70 Protein-Tyrosine Kinase; EC 2.7.10.2/src-Family Kinases; EC 3.1.3.48/Antigens, CD45; EC 3.1.3.48/Protein Tyrosine Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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