Document Detail

Stimulation of syncytium formation in vitro in human trophoblast cells by galectin-1.
MedLine Citation:
PMID:  20656349     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Galectin-1 (gal-1), a member of the mammalian beta-galactoside-binding proteins, exerts biological effects by recognition of glycan ligands, including those involved in cell adhesion and growth regulation. In trophoblast cells, gal-1 binds to cell surface glycoproteins (e.g., Mucin1). It has been demonstrated that gal-1 recognizes appropriate glycotopes on the syncytiotrophoblast and extravillous trophoblast from second trimester human placenta and choriocarcinoma cells BeWo, which reveal two coexisting phenotypes, the cytotrophoblast-like and the syncytiotrophoblast-like phenotype. So the aim of this study was to investigate the effect of gal-1 on syncytium formation in BeWo and human villous trophoblasts (HVT) cells.
MATERIALS AND METHODS: The effect of gal-1 on syncytium formation was investigated with immunocytochemical and double immunofluorescence stainings, cell-labelling and Real-time RT-PCR. BeWo choriocarcinoma and HVT cells were incubated in vitro for 24 and 48 h in the absence (controls) and presence of gal-1 and forskolin and stained with antibodies against Ki67, beta-catenin, E-cadherin and syncytin. BeWo and HVT cells were incubated with 60 microg/ml gal-1 for 48 h (BeWo) or 96 h (HVT) and cell fusion was detected by fluorescent cell-labelling solution. Finally, BeWo cells were incubated for 1 h or 48 h in the absence and presence of 60 microg/ml gal-1 and Real-time RT-PCR was performed.
RESULTS: We showed with immunocytochemical staining a downregulation of beta-catenin expression in the 24 h BeWo cell culture and with double immunofluorescence staining an inhibition of the beta-catenin and E-cadherin expression in the 48 h BeWo cell culture stimulated with gal-1 or forskolin. The inhibition of E-cadherin was demonstrated on mRNA level in the 1 h BeWo cell culture too. Increased cell fusion was also showed with DiO and DiI fluorescent cell-labelling solution in the 48 h BeWo cell culture. In addition, we demonstrated the downregulation of Ki67 protein expression in the 24 h BeWo cell culture and on mRNA level in the 1 h BeWo cell culture. We also showed the upregulation of syncytin protein and mRNA expression after incubation of the 48 h BeWo cell culture with gal-1 or forskolin. Similar results were obtained with HVT cells: the amount of cell fusion was significantly increased in the gal-1 treated 48 h HVT cell culture in vitro compared to untreated cells as demonstrated with beta-catenin and E-cadherin double immunofluorescence staining. This increase was also shown by fluorescent cell-labelling with DiO and DiI in the 96 h HVT cell culture compared to untreated cells.
CONCLUSION: Our data suggest that gal-1 stimulates the syncytium formation in choriocarcinoma cells BeWo and HVT cells in vitro and inhibits the expression of beta-catenin, E-cadherin and in addition Ki67 in BeWo cells. Therefore gal-1 may be a major trigger for the process of trophoblast cell fusion.
I Fischer; S Redel; S Hofmann; C Kuhn; K Friese; H Walzel; U Jeschke
Related Documents :
19504249 - Pancreatic remodeling: beta-cell apoptosis, proliferation and neogenesis, and the measu...
18223299 - Involvement of pi3k/akt pathway in tgf-beta2-mediated epithelial mesenchymal transition...
8254819 - Influence of growth factors on in vitro invasiveness and type iv collagenolysis of huma...
16505219 - The mesenchyme controls the timing of pancreatic beta-cell differentiation.
17581699 - Densin and beta-catenin form a complex and co-localize in cultured podocyte cell juncti...
15931009 - Role of hensin in mediating the adaptation of the cortical collecting duct to metabolic...
19083769 - Identification and isolation of male cells using fluorescence in situ hybridisation and...
20732049 - The effect of desferrioxamine on cell proliferation in human tumour cell lines.
7796839 - Treatment of amyotrophic lateral sclerosis using a gene therapy approach.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-24
Journal Detail:
Title:  Placenta     Volume:  31     ISSN:  1532-3102     ISO Abbreviation:  Placenta     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-06     Completed Date:  2010-12-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  825-32     Citation Subset:  IM    
Copyright Information:
Copyright 2010. Published by Elsevier Ltd.
Ludwig Maximilians University of Munich, Department of Obstetrics and Gynecology, Maistrasse 11, 80337 Munich, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cadherins / antagonists & inhibitors
Cell Fusion
Cell Line, Tumor
Forskolin / pharmacology
Galectin 1 / pharmacology*,  physiology
Gene Products, env / biosynthesis
Giant Cells / drug effects*,  metabolism
Pregnancy Proteins / biosynthesis
Trophoblasts / cytology*
Reg. No./Substance:
0/Cadherins; 0/Galectin 1; 0/Gene Products, env; 0/Pregnancy Proteins; 0/syncytin; 66428-89-5/Forskolin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Development of an ultrasound-responsive and mannose-modified gene carrier for DNA vaccine therapy.
Next Document:  Nine-year results of a volunteer lay network photoscreening program of 147 809 children using a phot...