| Stimulation of Na(+)-K(+)-ATPase by thyrotropin in cultured thyroid follicular cells. | |
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MedLine Citation:
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PMID: 7762619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thyroid-stimulating hormone (TSH; thyrotropin) produces a pleiotropic response in the thyroid gland, accelerating nearly every aspect of metabolic turnover within the follicular epithelia. We examined the effects of TSH on expression of Na(+)-K(+)-ATPase in FRTL-5 cells, a cell line derived from rat thyroid. TSH (10 mU/ml) produced a nearly twofold increase in abundance of the mRNA encoding the catalytic alpha 1-subunit within 6 h of treatment. With the four mRNAs encoding the beta 1-subunit, TSH produced a striking increase in abundance, but this regulation was discoordinate, and some species increased more than others. Similar increases in mRNA abundance were elicited by activators of the adenosine 3',5'-cyclic monophosphate second messenger system. In contrast to the alpha 1- and beta 1-mRNAs, the abundance of the mRNA encoding the beta 2-subunit was unchanged with TSH after 6 h, indicating that the effects of thyrotropin were not universal or indiscriminate. Thyrotropin also caused a 76% increase in Na(+)-K(+)-ATPase activity and a 46% increase in pump-mediated transport after 48 h. These studies suggest that the changes in metabolic turnover initiated by TSH during hormone synthesis include upregulation of the N(+)-K+ pump. |
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Authors:
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T A Pressley; S C Higham; L A Joson; D W Mercer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 268 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1995 May |
Date Detail:
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Created Date: 1995-06-29 Completed Date: 1995-06-29 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C1252-8 Citation Subset: IM |
Affiliation:
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Department of Physiology and Cell Biology, University of Texas Medical School, Houston 77030, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport, Active / drug effects Cells, Cultured Iodine / pharmacokinetics Ions RNA, Messenger / metabolism Rats Rats, Inbred F344 Sodium-Potassium-Exchanging ATPase / genetics, metabolism* Thyroid Gland / cytology, enzymology* Thyrotropin / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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HL-02305/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ions; 0/RNA, Messenger; 7553-56-2/Iodine; 9002-71-5/Thyrotropin; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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