Document Detail


Stimulation of Na(+)-H+ exchange, the Na(+)-K+ pump and Na+,K+,Cl- cotransport by endothelin-1 in cultured vascular smooth muscle cells.
MedLine Citation:
PMID:  2561135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Endothelin-1 concentrations higher than 10(-10) mol/l were able to strongly stimulate Na(+)-H+ exchange, the Na(+)-K+ pump and the Na+,K+Cl- cotransport system in A10 vascular smooth muscle cells. The stimulation of Na(+)-H+ exchange was more marked in fresh than in H(+)-loaded cells, suggesting an increase in the apparent affinity for internal H+. Pump stimulation appeared to be secondary to sodium entry through Na(+)-H+ exchange because it was absent in (1) Na(+)-loaded cells and (2) in the presence of ethyl-isopropylamiloride (EIPA). Stimulation of cotransport was blocked by indomethacin, suggesting the involvement of a cyclo-oxygenase product. In conclusion, the action of endothelin-1 in vascular smooth muscle induces the onset of negative feedback mechanisms which enhance the ability of the vascular cells to regulate disturbances in Na+,K+ and Cl- contents.
Authors:
C Rosati; E Jeanclos; C Nazaret; P E Chabrier; P Hannaert; P Braquet; R Garay
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of hypertension. Supplement : official journal of the International Society of Hypertension     Volume:  7     ISSN:  0952-1178     ISO Abbreviation:  J Hypertens Suppl     Publication Date:  1989 Dec 
Date Detail:
Created Date:  1990-05-11     Completed Date:  1990-05-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8501422     Medline TA:  J Hypertens Suppl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  S138-9     Citation Subset:  IM    
Affiliation:
INSERM U 7/CNRS UA 318, Hôpital Necker, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Biological Transport / drug effects,  physiology
Cell Line / drug effects,  metabolism
Cells, Cultured / drug effects,  metabolism
Chlorine / metabolism*
Dose-Response Relationship, Drug
Endothelins
Endothelium, Vascular / physiology*
Humans
Muscle, Smooth, Vascular / drug effects*,  metabolism
Peptides / pharmacology*
Potassium / metabolism*
Potassium Channels / drug effects*,  metabolism
Sodium / metabolism*
Sodium Channels / drug effects*,  metabolism
Stimulation, Chemical
Chemical
Reg. No./Substance:
0/Endothelins; 0/Peptides; 0/Potassium Channels; 0/Sodium Channels; 7440-09-7/Potassium; 7440-23-5/Sodium; 7782-50-5/Chlorine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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