Document Detail

Stimulation of L-asparate beta-decarboxylase formation by L-glutamate in Pseudomonas dacunhae and Improved production of L-alanine.
MedLine Citation:
PMID:  533269     Owner:  NLM     Status:  MEDLINE    
The formation of L-asparate beta-decarboxylase by Pseudomonas dacunhae was compared on media containing a variety of organic acids and amino acids as a carbon source. Although the enzyme was formed constitutively when the organism was grown on basal medium or on that containing tricarboxylic acid cycle intermediates, it was induced twofold by L-glutamate and repressed one-tenth by L-serine. L-Glutamine, L-proline, L-leucine, glycine, and L-threonine also showed induction effects lower than that of L-glutamate. L-Glutamate derepressed the serine effect. This glutamate effect was observed effect was observed with other microoganisms, e.g., Achromobacter pestifer and Achromobacter liquidum. Since the intermediates from L-glutamate metabolism had no effect, this induction effect was specific to L-glutamate. The formation of some glutamate-related enzymes was measured and is discussed in relation to the formation of L-asparate beta-decarboxylase. L-Asparate beta-decarboxylase was purified to an electrophoretically homogenous state from L-glutamate-grown cells of P. dacunhae, and some properties were compared with those of the enzyme from fumarate-grown cells. The two enzymes were identical in disc electrophoresis, molecular weight, and some enzymatic properties. The industrial production of L-alanine from L-aspartic acid acid was improved by using the culture broth with highly induced L-asparate beta-decarboxylase (9.4 U/ml of broth).
T Shibatani; T Kakimoto; I Chibata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  38     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1979 Sep 
Date Detail:
Created Date:  1980-04-25     Completed Date:  1980-04-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  359-64     Citation Subset:  IM    
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MeSH Terms
Alanine / biosynthesis*
Amino Acids / metabolism
Carboxy-Lyases / biosynthesis*,  isolation & purification
Fumarates / metabolism
Glutamates / metabolism*
Pseudomonas / metabolism*
Reg. No./Substance:
0/Amino Acids; 0/Fumarates; 0/Glutamates; 56-41-7/Alanine; EC 4.1.1.-/Carboxy-Lyases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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