Document Detail


Stimulation of L-asparate beta-decarboxylase formation by L-glutamate in Pseudomonas dacunhae and Improved production of L-alanine.
MedLine Citation:
PMID:  533269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The formation of L-asparate beta-decarboxylase by Pseudomonas dacunhae was compared on media containing a variety of organic acids and amino acids as a carbon source. Although the enzyme was formed constitutively when the organism was grown on basal medium or on that containing tricarboxylic acid cycle intermediates, it was induced twofold by L-glutamate and repressed one-tenth by L-serine. L-Glutamine, L-proline, L-leucine, glycine, and L-threonine also showed induction effects lower than that of L-glutamate. L-Glutamate derepressed the serine effect. This glutamate effect was observed effect was observed with other microoganisms, e.g., Achromobacter pestifer and Achromobacter liquidum. Since the intermediates from L-glutamate metabolism had no effect, this induction effect was specific to L-glutamate. The formation of some glutamate-related enzymes was measured and is discussed in relation to the formation of L-asparate beta-decarboxylase. L-Asparate beta-decarboxylase was purified to an electrophoretically homogenous state from L-glutamate-grown cells of P. dacunhae, and some properties were compared with those of the enzyme from fumarate-grown cells. The two enzymes were identical in disc electrophoresis, molecular weight, and some enzymatic properties. The industrial production of L-alanine from L-aspartic acid acid was improved by using the culture broth with highly induced L-asparate beta-decarboxylase (9.4 U/ml of broth).
Authors:
T Shibatani; T Kakimoto; I Chibata
Related Documents :
20634929 - The resistance to freeze-drying and to storage was determined as the cellular ability t...
2906529 - The neuroexcitotoxic amino acids glutamate and aspartate are altered in the spinal cord...
4528109 - Vitamin k dependent modifications of glutamic acid residues in prothrombin.
10743819 - Excitatory amino acid profiles of synovial fluid from patients with arthritis.
2599979 - Effects of monensin and ph on the production and utilization of pyro-glutamate, a novel...
2161549 - Amino acid patterns in schizophrenia: some new findings.
20435419 - Fatty acid profiles in meat from caiman yacare (caiman crocodilus yacare) raised in the...
7546229 - Relationships between microflora and caecal fermentation in rabbits before and after we...
9145199 - Protective effects of hydroxybenzoic acids and their esters on cell damage induced by h...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  38     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1979 Sep 
Date Detail:
Created Date:  1980-04-25     Completed Date:  1980-04-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  359-64     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Alanine / biosynthesis*
Amino Acids / metabolism
Carboxy-Lyases / biosynthesis*,  isolation & purification
Fermentation
Fumarates / metabolism
Glutamates / metabolism*
Pseudomonas / metabolism*
Stereoisomerism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fumarates; 0/Glutamates; 56-41-7/Alanine; EC 4.1.1.-/Carboxy-Lyases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  In vitro activity of LY127935, a new 1-oxa cephalosporin, against aerobic gram-negative bacilli.
Next Document:  Specificity of bacteriolytic enzyme II from a soil amoeba, Hartmannella glebae.