Document Detail

Stimulation of Escherichia coli DNA damage inducible DNA helicase DinG by the single-stranded DNA binding protein SSB.
MedLine Citation:
PMID:  23036643     Owner:  NLM     Status:  MEDLINE    
Escherichia coli DNA damage inducible protein DinG is a superfamily II DNA helicase and is closely related to human DNA helicase XPD. Here, we report that E. coli single-stranded DNA binding protein (SSB) is able to form a stable protein complex with DinG and to stimulate the DinG DNA helicase activity. An SSB mutant that retains the single-stranded DNA binding activity but fails to form a protein complex with DinG becomes a potent inhibitor for the DinG DNA helicase, suggesting that E. coli wild-type SSB stimulates the DinG DNA helicase via specific protein-protein interaction.
Zishuo Cheng; Aimee Caillet; Binbin Ren; Huangen Ding
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  FEBS letters     Volume:  586     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-29     Completed Date:  2013-01-03     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3825-30     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
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MeSH Terms
Chromatography, Gel
DNA Damage
DNA, Single-Stranded / genetics,  metabolism*
DNA-Binding Proteins / genetics,  metabolism*
Escherichia coli / genetics*,  metabolism
Escherichia coli Proteins / genetics,  metabolism*
Protein Binding
Sequence Homology, Amino Acid
Transformation, Bacterial
Xeroderma Pigmentosum Group D Protein / genetics,  metabolism
Grant Support
R01 CA107494/CA/NCI NIH HHS; R01CA107494/CA/NCI NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/DNA, Single-Stranded; 0/DNA-Binding Proteins; 0/Escherichia coli Proteins; 0/SSB protein, E coli; 0/dinG protein, E coli; EC 3.6.1.-/Xeroderma Pigmentosum Group D Protein; EC 5.99.-/ERCC2 protein, human

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