Document Detail

Stimulation of DNA synthesis in human fibroblasts by thrombin.
MedLine Citation:
PMID:  206567     Owner:  NLM     Status:  MEDLINE    
Earlier work showed that thrombin stimulates proliferation of human fibroblasts in serumfree medium. This work demonstrates (1) that thrombin has to be prensent during most or all of the G1 period to ensure maximal DNA synthesis, (2) that DNA synthesis increases about three hours later after thrombin than after serum treatment, (3) that both thrombin and serum activate transport of uridine, D--2-deoxy-glucose and putrescine, (4) that thrombin is able to increase 3H-thymidine incorporation also in SV40 transformed human fibroblasts, in HeLa cells and in two continuous monkey cell lines.
P Pohjanpelto
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  95     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1978 May 
Date Detail:
Created Date:  1978-07-15     Completed Date:  1978-07-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  189-94     Citation Subset:  IM    
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MeSH Terms
Cell Line
DNA / biosynthesis*
Deoxyglucose / metabolism
Fibroblasts / metabolism
Hela Cells / metabolism
Lung / embryology
Putrescine / metabolism
Simian virus 40
Species Specificity
Stimulation, Chemical
Thrombin / pharmacology*
Uridine / metabolism
Reg. No./Substance:
110-60-1/Putrescine; 154-17-6/Deoxyglucose; 58-96-8/Uridine; 9007-49-2/DNA; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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