| Stimulation of DNA synthesis in human fibroblasts by thrombin. | |
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MedLine Citation:
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PMID: 206567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Earlier work showed that thrombin stimulates proliferation of human fibroblasts in serumfree medium. This work demonstrates (1) that thrombin has to be prensent during most or all of the G1 period to ensure maximal DNA synthesis, (2) that DNA synthesis increases about three hours later after thrombin than after serum treatment, (3) that both thrombin and serum activate transport of uridine, D--2-deoxy-glucose and putrescine, (4) that thrombin is able to increase 3H-thymidine incorporation also in SV40 transformed human fibroblasts, in HeLa cells and in two continuous monkey cell lines. |
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Authors:
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P Pohjanpelto |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of cellular physiology Volume: 95 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1978 May |
Date Detail:
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Created Date: 1978-07-15 Completed Date: 1978-07-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 189-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line DNA / biosynthesis* Deoxyglucose / metabolism Fibroblasts / metabolism Haplorhini Hela Cells / metabolism Humans Kidney Lung / embryology Putrescine / metabolism Simian virus 40 Species Specificity Stimulation, Chemical Thrombin / pharmacology* Uridine / metabolism |
| Chemical | |
Reg. No./Substance:
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110-60-1/Putrescine; 154-17-6/Deoxyglucose; 58-96-8/Uridine; 9007-49-2/DNA; EC 3.4.21.5/Thrombin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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