Document Detail


Stimulation of bacterial DNA synthesis by algal exudates in attached algal-bacterial consortia.
MedLine Citation:
PMID:  16347218     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Algal-bacterial consortia attached to polystyrene surfaces were prepared in the laboratory by using the marine diatom Amphora coffeaeformis and the marine bacterium Vibrio proteolytica (the approved name of this bacterium is Vibrio proteolyticus [W. E. C. Moore, E. P. Cato, and L. V. H. Moore, Int. J. Syst. Bacteriol. 35:382-407, 1985]). The organisms were attached to the surfaces at cell densities of approximately 5 x 10 cells cm (diatoms) and 5 x 10 cells cm (bacteria). The algal-bacterial consortia consistently exhibited higher rates of [H]thymidine incorporation than did biofilms composed solely of bacteria. The rates of [H]thymidine incorporation by the algal-bacterial consortia were fourfold greater than the rates of incorporation by monobacterial biofilms 16 h after biofilm formation and were 16-fold greater 70 h after biofilm formation. Extracellular material released from the attached Amphora cells supported rates of bacterial activity (0.8 x 10 to 17.9 x 10 mol of [H]thymidine incorporated cell h) and growth (doubling time, 29.5 to 1.4 days) comparable to values reported for a wide variety of marine and freshwater ecosystems. In the presence of sessile diatom populations, DNA synthesis by attached V. proteolytica cells was light dependent and increased with increasing algal abundance. The metabolic activity of diatoms thus appears to be the rate-limiting process in biofilm development on illuminated surfaces under conditions of low bulk-water dissolved organic carbon.
Authors:
R E Murray; K E Cooksey; J C Priscu
Related Documents :
11705908 - Induction of epithelial cell death including apoptosis by enteropathogenic escherichia ...
15577928 - Survival of protozoan intracellular parasites in host cells.
11290328 - Control of cell shape in bacteria: helical, actin-like filaments in bacillus subtilis.
3109968 - Ultrastructural characterization of leucocytes in the pronephros of carp (cyprinus carp...
19779498 - Novel roles for gapdh in cell death and carcinogenesis.
16045548 - B-1 cells are pivotal for in vivo inflammatory giant cell formation.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  52     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1986 Nov 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-07-02     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1177-82     Citation Subset:  -    
Affiliation:
Department of Microbiology and Department of Biology, Montana State University, Bozeman, Montana 59717.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Isolation of Pathogenic Bacillus circulans from Callus Cultures and Healthy Offshoots of Date Palm (...
Next Document:  Bacteriocinlike Activity within the Genus Thermus.