| Stimulating Cardiac Muscle by Light: Cardiac Optogenetics by Cell Delivery. | |
| | |
MedLine Citation:
|
PMID: 21828312 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND: -After the recent cloning of light-sensitive ion channels and their expression in mammalian cells, a new field, optogenetics, emerged in neuroscience, allowing for precise perturbations of neural circuits by light. However, functionality of optogenetic tools has not been fully explored outside neuroscience; and a non-viral, non-embryogenesis based strategy for optogenetics has not been shown before. METHODS AND RESULTS: -We demonstrate the utility of optogenetics to cardiac muscle by a tandem cell unit (TCU) strategy, where non-excitable cells carry exogenous light-sensitive ion channels, and when electrically coupled to cardiomyocytes, produce optically-excitable heart tissue. A stable channelrhodopsin2 (ChR2) expressing cell line was developed, characterized and used as a cell delivery system. The TCU strategy was validated in vitro in cell pairs with adult canine myocytes (for a wide range of coupling strengths) and in cardiac syncytium with neonatal rat cardiomyocytes. For the first time, we combined optical excitation and optical imaging to capture light-triggered muscle contractions and high-resolution propagation maps of light-triggered electrical waves, found to be quantitatively indistinguishable from electrically-triggered waves. CONCLUSIONS: -Our results demonstrate feasibility to control excitation and contraction in cardiac muscle by light using the TCU approach. Optical pacing in this case uses less energy, offers superior spatiotemporal control, remote access and can serve not only as an elegant tool in arrhythmia research, but may form the basis for a new generation of light-driven cardiac pacemakers and muscle actuators. The TCU strategy is extendable to (non-viral) stem cell therapy and is directly relevant to in vivo applications. |
| | |
Authors:
|
Zhiheng Jia; Virginijus Valiunas; Zongju Lu; Harold Bien; Huilin Liu; Hong-Zhang Wang; Barbara Rosati; Peter R Brink; Ira S Cohen; Emilia Entcheva |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-8-9 |
Journal Detail:
|
Title: Circulation. Arrhythmia and electrophysiology Volume: - ISSN: 1941-3084 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
|
Created Date: 2011-8-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101474365 Medline TA: Circ Arrhythm Electrophysiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Stony Brook University, Stony Brook, NY. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Turkish Dental Students' and Dentists' Ability to Assess Gingival Health Status with DAAGS Software.
Next Document: Sleep-disordered breathing, hypoxia, and risk of mild cognitive impairment and dementia in older wom...