| Stiffness and shortening changes in myofilament-extracted rat cardiac myocytes. | |
| | |
MedLine Citation:
|
PMID: 2916687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Sarcomere lengths, cell widths, volumes, stiffness, and regional striation uniformity were determined from isolated adult cardiac myocytes. Single cells were examined in the control saline solution followed by a sequence of relaxing, membrane skinning, and myofilament extraction solutions. Cell size and shape parameters were determined from freely dispersed myocytes, whereas stiffness was measured from myocytes attached to a perturbator and tension transducer with micropipettes. There were small changes in cell appearance, size, shape, and stiffness in the relaxing and skinning solutions. However, in 0.17-0.56 M KCl myosin extraction media, cell length declined significantly to 1.19 microns, and stiffness fell to 5-10% of control. The rate of cell shortening and stiffness decline was dependent on KCl concentration and pH. Subsequent exposure to higher ionic strength 0.60 M KI thin filament extraction media elicited additional decreases in stiffness (less than 5% of control) and cell length (0.98 micron). Cell shortening and stiffness decline have similar time courses under the same conditions, and they appear to coincide with A-band disassembly as indicated by electron micrographs. These data suggest that cardiac myocyte stiffness, size, and shape are determined in part by a stressed cytoskeleton that is associated with the myofilament apparatus. |
| | |
Authors:
|
K P Roos; A J Brady |
Related Documents
:
|
6650117 - Volume changes during mitosis in pancreatic acinar cells of the rat. 7548147 - The effect of lethal acid stress on na+/h+ exchanger isoforms in cultured inner medulla... 8498197 - Functional anatomy of human speech. 9149237 - Giardia intestinalis: volume recovery in response to cell swelling. 19159517 - Induction of hair follicle regeneration in rat ear by microencapsulated human hair derm... 1618697 - Flow cytometric bromodeoxyuridine/dna analysis of hyperthermia and/or adriamycin for hu... |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The American journal of physiology Volume: 256 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1989 Feb |
Date Detail:
|
Created Date: 1989-03-23 Completed Date: 1989-03-23 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: H539-51 Citation Subset: IM |
Affiliation:
|
Department of Physiology, School of Medicine, University of California, Los Angeles 90024-1760. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cytoskeleton / physiology* Heart / physiology* Microfilaments / drug effects, physiology*, ultrastructure Microscopy, Electron Myocardial Contraction* Myofibrils / physiology* Myosins / analysis, physiology Potassium Chloride / pharmacology Rats Sarcomeres / drug effects, physiology*, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
|
HL-29671/HL/NHLBI NIH HHS; HL-30828/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
7447-40-7/Potassium Chloride; EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of hemoglobin concentration on critical cardiac output and oxygen transport.
Next Document: Effects of ATP precursors on ATP and free ADP content and functional recovery of postischemic hearts...