Document Detail

Stiffness, compliance, elasticity and force generation of outer hair cells.
MedLine Citation:
PMID:  1604988     Owner:  NLM     Status:  MEDLINE    
Isolated outer hair cells (OHCs) were partially sucked into especially designed cell capillaries allowing an experimental reconstitution of the cells' electroanatomy. The experimental approach separated the apical from the basolateral parts of the cells thus forming an artificial scala media and scala tympani. Resistance between both was 121 +/- 42 M omega. A sequence of negative and positive pressures was applied to the basal cell pole allowing "pulling" or "pushing" of the sensory cell investigated. The resulting length changes together with the known pressures allowed the estimation of an actual longitudinal compliance of 354 +/- 35 m/N. Following "pulling" OHCs tended to resume their initial shape after the force had ceased to be effective indicating elastic distortions. The calculated elasticity modulus of OHCs amounted to 6.1 +/- 3.4 kN/m2. From this data an actual longitudinal whole cell stiffness of OHCs of 3 x 10(-3) N/m was calculated. Ultrasound scanning of immobilized OHCs identified the cuticular plate (CP) and a central core between CP and basal cell pole as structures contributing to the cells' acoustic stiffness. Changes of the potential differences between the artificial scala media and scala tympani resulted in active length changes following the command voltage with a slope of delta 1/(1 x U) = 0.055 V-1. Assuming the validity of Hooke's law, the force generation associated with the active length changes can be calculated since the compliance is known.(ABSTRACT TRUNCATED AT 250 WORDS)
H P Zenner; A H Gitter; M Rudert; A Ernst
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  112     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  1992  
Date Detail:
Created Date:  1992-07-15     Completed Date:  1992-07-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  248-53     Citation Subset:  IM    
Department of Otolaryngology, University of Tübingen, Germany.
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MeSH Terms
Basilar Membrane / physiology*
Cochlear Microphonic Potentials / physiology*
Guinea Pigs
Hair Cells, Auditory / physiology*
Organ of Corti / physiology*

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