Document Detail


Stiffening of human skin fibroblasts with age.
MedLine Citation:
PMID:  22099845     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Changes in mechanical properties are an essential characteristic of the aging process of human skin. Previous studies attribute these changes predominantly to the altered collagen and elastin organization and density of the extracellular matrix. Here, we show that individual dermal fibroblasts also exhibit a significant increase in stiffness during aging in vivo. With the laser-based optical cell stretcher we examined the viscoelastic biomechanics of dermal fibroblasts isolated from 14 human donors aged 27 to 80. Increasing age was clearly accompanied by a stiffening of the investigated cells. We found that fibroblasts from old donors exhibited an increase in rigidity of ∼60% with respect to cells of the youngest donors. A FACS analysis of the content of the cytoskeletal polymers shows a shift from monomeric G-actin to polymerized, filamentous F-actin, but no significant changes in the vimentin and microtubule content. The rheological analysis of fibroblast-populated collagen gels demonstrates that cell stiffening directly results in altered viscoelastic properties of the collagen matrix. These results identify a new mechanism that may contribute to the age-related impairment of elastic properties in human skin. The altered mechanical behavior might influence cell functions involving the cytoskeleton, such as contractility, motility, and proliferation, which are essential for reorganization of the extracellular matrix.
Authors:
Christian Schulze; Franziska Wetzel; Thomas Kueper; Anke Malsen; Gesa Muhr; Soeren Jaspers; Thomas Blatt; Klaus-Peter Wittern; Horst Wenck; Josef A Käs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinics in plastic surgery     Volume:  39     ISSN:  1558-0504     ISO Abbreviation:  Clin Plast Surg     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-11-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0424767     Medline TA:  Clin Plast Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9-20     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Division of Soft Matter Physics, Department of Physics, University of Leipzig, Leipzig, Germany; R&D, Beiersdorf, Hamburg, Germany.
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