Document Detail


Sticky windows: chemical and biological characteristics of the organic film derived from particulate and gas-phase air contaminants found on an urban impervious surface.
MedLine Citation:
PMID:  12712271     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel environmentally derived mixture that integrates exposure to atmospherically derived gas- and particle-phase compounds in urban areas-namely, the organic film that develops as a thin layer on urban impervious surfaces-was investigated for its ability to induce gene expression via the aryl hydrocarbon receptor (AhR). The organic film on window glass from 21 sites in downtown Toronto (Ontario, Canada) was found to contain a complex mixture of environmental contaminants typical of urban environments, notably PAHs, n-alkanes, PCBs, organochlorine (OC) pesticides, and polar constituents. Using a stably transfected reporter cell line, we found that the crude extract of organic film induces AhR-dependent gene expression in a dose-dependent fashion. Three subfractions of the crude extract induced significant luciferase expression: nonpolar aromatic > polar aromatic > nonpolar aliphatic. Recombination of the fractions did not lead to recovery of the full activity of the crude extract, which may indicate that some of the compounds lost during fractionation were significant contributors to the induction observed with the crude extract. The interactions between a tonic dose of B[ a]P (10(-7) M) and each of the aromatic fractions were determined to be antagonistic following analysis by the method of isoboles. Our results suggest that organic film makes up a diverse array of compounds active at the AhR and that these compounds may not interact in a strictly additive manner.
Authors:
E M Hodge; M L Diamond; B E McCarry; G A Stern; P A Harper
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of environmental contamination and toxicology     Volume:  44     ISSN:  0090-4341     ISO Abbreviation:  Arch. Environ. Contam. Toxicol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-24     Completed Date:  2003-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0357245     Medline TA:  Arch Environ Contam Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  421-9     Citation Subset:  IM    
Affiliation:
Department of Geography, University of Toronto, Ontario, Canada M5S 3G3.
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MeSH Terms
Descriptor/Qualifier:
Air Pollutants / toxicity*
Animals
Cities
Dose-Response Relationship, Drug
Drug Interactions
Gene Expression / drug effects*
Genes, Reporter / genetics
Glass / chemistry*
Luciferases / genetics
Mice
Ontario
Organic Chemicals / toxicity*
Receptors, Aryl Hydrocarbon / genetics*
Surface Properties
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Air Pollutants; 0/Organic Chemicals; 0/Receptors, Aryl Hydrocarbon; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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