Document Detail


Sterol carrier protein-2 localization in endoplasmic reticulum and role in phospholipid formation.
MedLine Citation:
PMID:  11003606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although sterol carrier protein-2 (SCP-2; also called nonspecific lipid transfer protein) binds fatty acids and fatty acyl-CoAs, its role in fatty acid metabolism is not fully understood. L-cell fibroblasts stably expressing SCP-2 were used to resolve the relationship between SCP-2 intracellular location and fatty acid transacylation in the endoplasmic reticulum. Indirect immunofluorescence double labeling and laser scanning confocal microscopy detected SCP-2 in peroxisomes > endoplasmic reticulum > mitochondria > lysosomes. SCP-2 enhanced incorporation of exogenous [(3)H]oleic acid into phospholipids and triacylglycerols of overexpressing cells 1.6- and 2.5-fold, respectively, stimulated microsomal incorporation of [1-(14)C]oleoyl-CoA into phosphatidic acid in vitro 13-fold, and exhibited higher specificity for unsaturated versus saturated fatty acyl-CoA. SCP-2 enhanced the rate-limiting step in microsomal phosphatidic acid biosynthesis mediated by glycerol-3-phosphate acyltransferase. SCP-2 also enhanced microsomal acyl-chain remodeling of phosphatidylethanolamine up to fivefold and phosphatidylserine twofold, depending on the specific fatty acyl-CoA, but had no effect on other phospholipid classes. In summary, these results were consistent with a role for SCP-2 in phospholipid synthesis in the endoplasmic reticulum.
Authors:
O Starodub; C A Jolly; B P Atshaves; J B Roths; E J Murphy; A B Kier; F Schroeder
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  279     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-12     Completed Date:  2000-10-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1259-69     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, Texas A&M University, College Station, Texas 77843-4466, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / metabolism
Acylation / drug effects
Animals
Carrier Proteins / genetics,  metabolism*,  pharmacology
Endoplasmic Reticulum / metabolism*
Fatty Acids / metabolism
Fibroblasts / cytology,  metabolism*
Glycerol-3-Phosphate O-Acyltransferase / metabolism
Immunohistochemistry
Intracellular Fluid / metabolism
L Cells (Cell Line)
Mice
Microscopy, Fluorescence
Microsomes / metabolism
Peroxisomes / metabolism
Phosphatidic Acids / biosynthesis
Phospholipids / biosynthesis*
Plant Proteins*
Rabbits
Substrate Specificity
Transfection
Triglycerides / biosynthesis
Grant Support
ID/Acronym/Agency:
DK41402/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Carrier Proteins; 0/Fatty Acids; 0/Phosphatidic Acids; 0/Phospholipids; 0/Plant Proteins; 0/Triglycerides; 0/sterol carrier proteins; EC 2.3.1.15/Glycerol-3-Phosphate O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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