| Sterol carrier protein-2 expression alters sphingolipid metabolism in transfected mouse L-cell fibroblasts. | |
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MedLine Citation:
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PMID: 16444586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of sterol carrier protein-2 (SCP-2) on the cellular metabolism of sphingolipids was examined in control mouse L-cells and stably transfected clones expressing the protein SCP-2. Three approaches were used to examine for differences; (1) compositional analysis of endogenous sphingolipid classes, (2) metabolism of NBD-ceramide, and (3) live cell labelling via endocytic uptake of BODIPY-sphingomyelin. SCP-2 over expression significantly altered the endogenous levels of both neutral and acidic sphingolipid classes. Among the neutral sphingolipids, expression of SCP-2 induced a 1.7-fold increase in the level of lactosylceramide (LacCer, p < 0.05) and a similar fold decrease in the level of the higher-order neutral glycosylceramides (p < 0.05). Among the acidic sphingolipids, SCP-2 resulted in a 5.2-fold decrease in the endogenous plasma membrane level of ganglioside GM1 (p < 0.03). Incubation of both control and transfected cell lines with NBD-ceramide resulted in the rapid establishment of a steady-state distribution of NBD-labelled sphingomyelin (NBD-SM) and glucosylceramide (NBD-GlcCer). In the SCP-2 expressing clones the conversion of NBD-Cer to NBD-GlcCer was 30% lower during incubation periods between 5 and 30 min (p < 0.025). Inspection of the cells by fluorescence microscopy after incubation with BODIPY labelled sphingomyelin (BODIPY-SM) revealed similar punctuated patterns with no distinguishable differences between the cell types. These results imply that SCP-2 plays a role in modulating enzymatic steps involved in metabolism of sphingolipid homeostasis. |
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Authors:
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Daniel G Milis; Messiah K Moore; Barbara P Atshaves; Friedhelm Schroeder; John R Jefferson |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 283 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-01-30 Completed Date: 2006-04-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 57-66 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Luther College, Decorah, Iowa, 52101-1045, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4-Chloro-7-nitrobenzofurazan
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analogs & derivatives,
pharmacokinetics,
pharmacology Animals Biological Transport Carrier Proteins / metabolism* Cell Membrane / metabolism Ceramides / pharmacology Fibroblasts / metabolism* G(M1) Ganglioside / analogs & derivatives, metabolism Glucosylceramides / pharmacokinetics Homeostasis L Cells (Cell Line) Mice Microscopy, Fluorescence Sphingolipids / metabolism* Sphingomyelins / pharmacokinetics Transfection |
| Grant Support | |
ID/Acronym/Agency:
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GM31651/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Ceramides; 0/Glucosylceramides; 0/N-4-nitrobenzo-2-oxa-1,3-diazoleaminocaproyl sphingosylphosphorylcholine; 0/Sphingolipids; 0/Sphingomyelins; 0/ganglioside GM1alpha; 0/sterol carrier proteins; 10199-89-0/4-Chloro-7-nitrobenzofurazan; 37758-47-7/G(M1) Ganglioside; 86701-10-2/N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl sphingosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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