| Sterol carrier protein-2 expression alters phospholipid content and fatty acyl composition in L-cell fibroblasts. | |
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MedLine Citation:
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PMID: 10787439 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects sterol carrier protein-2 (SCP-2) expression on L-cell phospholipid levels and fatty acyl composition was assessed using L-cells transfected with the murine cDNA encoding for either the 15 kDa proSCP-2 or 13.2 kDa SCP-2. Expression of these proteins reduced total phospholipid mass (nmol/mg protein) by 24% and reduced the cholesterol to phospholipid ratio 60 and 28%, respectively. In 15 kDa proSCP-2 expressing cells, individual phospholipid class masses, excluding sphingomyelin (CerPCho), were reduced as follows: phosphatidylinositol (PtdIns) and phosphatidylserine (PtdSer) >> ethanolamine glycerophospholipid (EtnGpl) > choline glycerophospholipid (ChoGpl). Furthermore, ethanolamine plasmalogen mass was decreased 25%, while choline plasmalogen mass was elevated 30% in 15 kDa proSCP-2 expressing cells. In 13.2 kDa SCP-2 expressing cells, phospholipid class mass was decreased as follows: PtdIns and PtdSer >> ChoGpl. These changes in phospholipid mass resulted in altered cellular phospholipid composition. Expression of either protein differentially altered the type of fatty acid esterified onto the phospholipids. These effects included a greater proportion of polyunsaturated fatty acids and a reduction in saturated fatty acids, although 15 kDa proSCP-2 expression had a more robust effect on these parameters than did 13.2 kDa SCP-2 expression. In summary, expression of SCP-2 reduced individual phospholipid class mass, except for CerPCho, and altered the fatty acid composition of each phospholipid class examined. These results clearly demonstrate that SCP-2 expression altered basal phospholipid levels, suggesting that SCP-2 can alter the function of endoplasmic reticulum phospholipid synthetic enzymes. |
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Authors:
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E J Murphy; T Stiles; F Schroeder |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of lipid research Volume: 41 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-07-24 Completed Date: 2000-07-24 Revised Date: 2009-11-03 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 788-96 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Physiology, Texas A&M University, TVMC, College Station, TX 77843-4466, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins / genetics* Cholesterol / metabolism Endoplasmic Reticulum / metabolism Fatty Acids / metabolism* Gene Expression L Cells (Cell Line) Mice Peroxisomes / metabolism Phospholipids / classification, metabolism* Plant Proteins* Plasmalogens / metabolism Protein Precursors / genetics Sterols / metabolism* Transfection |
| Grant Support | |
ID/Acronym/Agency:
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DK41402/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Fatty Acids; 0/Phospholipids; 0/Plant Proteins; 0/Plasmalogens; 0/Protein Precursors; 0/Sterols; 0/sterol carrier proteins; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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