| Steroidogenesis in isolated adrenocortical cells during development in rats. | |
| | |
MedLine Citation:
|
PMID: 8386118 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
After postnatal day 1 (d1), the hypothalamo-pituitary-adrenal axis of neonatal rats becomes less responsive to certain stimuli for up to 2 weeks. The present study was designed to quantify the development of adrenocortical cell responsiveness to its normal secretagogue, adrenocorticotropic hormone (ACTH), and to better localize intracellular sites of adrenal cell hyporesponsivity. Maximum steroidogenic responses of collagenase-dispersed adrenocortical cells (using two isolation methods) to ACTH varied significantly in the order adult > d1 > d10. The response pattern to dibutyryl cAMP ((Bu)2cAMP) was identical to that observed for ACTH (adult > d1 > d10), suggesting that neonatal adrenal responsiveness is limited by a site distal to cAMP formation. Sensitivity (EC50) of adult cells to ACTH was approximately 3-fold greater than in neonatal cells, but there was no age-dependent shift in sensitivity to (Bu)2cAMP. 20 alpha-Hydroxycholesterol (20 alpha-OHCHOL), a membrane permeable analog of cholesterol, also failed to normalize the d10 adrenal response to ACTH. This result indicates that one site of refractoriness is apparently distal to cholesterol transport, and strongly suggests possible differential cytochrome P450 enzyme expression or activity in neonatal rat adrenal cells. Finally, although stimulated secretion was lower in neonatal cells, basal corticosterone secretion was significantly greater in neonatal adrenals, suggesting that constitutive activity of neonatal adrenal cells is high compared to that of adult cells. |
| | |
Authors:
|
M Arai; E P Widmaier |
Related Documents
:
|
7849108 - Cyclic amp regulation of annexins i, ii, v synthesis and localization in cultured porci... 6395958 - Ultrastructural immunocytochemical demonstration of d2-protein in adrenal medulla. 12448768 - Influence of lithium on growth and viability of thyroid follicular cells. 16934868 - (meth)acrylate monomer-induced cytotoxicity and intracellular ca(2+) mobilization in hu... 9576828 - Spontaneous transdifferentiation of quail pigmented epithelial cell is accompanied by a... 3955138 - Patterns of [3h] thymidine incorporation differ in immature rats and mature, cycling rats. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Molecular and cellular endocrinology Volume: 92 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 1993 Mar |
Date Detail:
|
Created Date: 1993-05-17 Completed Date: 1993-05-17 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 91-7 Citation Subset: IM |
Affiliation:
|
Department of Biology, Boston University, MA 02215. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Cortex
/
cytology,
drug effects,
growth & development,
metabolism* Adrenal Cortex Hormones / biosynthesis* Adrenocorticotropic Hormone / pharmacology Age Factors Animals Animals, Newborn Cells, Cultured Corticosterone / secretion Female Hydroxycholesterols / pharmacology Male Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
|
DK41263/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Adrenal Cortex Hormones; 0/Hydroxycholesterols; 50-22-6/Corticosterone; 516-72-3/20-hydroxycholesterol; 9002-60-2/Adrenocorticotropic Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Steroid action at the plasma membrane: progesterone stimulation of phosphatidylcholine-specific phos...
Next Document: Understanding cytochrome c function: engineering protein structure by semisynthesis.