Document Detail


Steroidogenesis and cAMP production in isolated avian granulosa cells during follicular maturation: lack of positive correlation.
MedLine Citation:
PMID:  3022519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Luteinizing hormone- and forskolin-induced acute steroidogenesis and cAMP production were studied in dispersed chicken granulosa cells in relation to follicular maturation. Cells isolated from the 6 largest preovulatory follicles (F1-F6) were used in this study. Basal steroidogenic activity increased with progressive maturation of the follicle without a corresponding rise in basal cAMP production. Both LH- and forskolin-promoted progesterone production was directly correlated with the maturational stage of the follicle and the dose of the agonists. On the other hand, LH caused the greatest increase in cAMP production in the third and fourth largest preovulatory follicles (F3-F4), whereas forskolin-stimulated cAMP accumulation was maximal in the least mature granulosa cells (F5-F6). Thereafter it decreased in proportion with follicular development. It is concluded that LH-induced acute steroidogenesis in chicken granulosa cells is not positively correlated with cAMP production during the last few days of follicular maturation. Therefore the steroidogenic responsiveness of granulosa cells of the largest follicle to LH cannot be ascribed solely to receptor-coupled adenylate cyclase activity. As shown by the forskolin experiment, the intrinsic activity of the adenylate cyclase-cAMP system is greatest in the small F5, F6 follicles which respond only minimally in terms of steroidogenesis to either a receptor dependent agonist (LH) or a nonreceptor agonist (forskolin).
Authors:
E K Asem; F Hertelendy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta endocrinologica     Volume:  113     ISSN:  0001-5598     ISO Abbreviation:  Acta Endocrinol.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-12-04     Completed Date:  1986-12-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370312     Medline TA:  Acta Endocrinol (Copenh)     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  289-97     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cyclic AMP / biosynthesis*
Female
Forskolin / pharmacology*
Granulosa Cells / metabolism*
Luteinizing Hormone / pharmacology*
Ovarian Follicle / physiology
Poultry
Progesterone / biosynthesis*
Chemical
Reg. No./Substance:
57-83-0/Progesterone; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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