Document Detail


Steroid resistance in nasal polyposis: role of glucocorticoid receptor and TGF-beta1.
MedLine Citation:
PMID:  23193535     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Glucocorticoids (GCs) are considered drugs of choice for treating nasal polyps (NPs). However, a subset of patients shows a limited clinical response even to high doses of GCs. Altered expression of glucocorticoid receptors (GRs), namely GR-alpha; and GR-beta;, is a potential mechanism underlying GC insensitivity. GCs modulate the expression of several cytokines, including transforming growth factor-beta (TGF-beta), which may contribute to cellular proliferation in NPs. The study investigates some biomolecular features of GC-resistant NPs, and examines possible differences from normal mucosa (NM).
METHODOLOGY: Radioligand binding assay (binding) was used to determine GR-alpha; binding capacity; Western blotting was used to evaluate GR-alpha;, GR-beta;, and TGF-beta; expression and GR-alpha; subcellular distribution. NPs were sampled in 32 patients during ethmoidectomy; NM was taken from 15 healthy patients during rhinoplasty.
RESULTS: GR-alpha; was present in NPs and NM, with lower affinity for the ligand in NPs. GR-alpha; was prevalent in the cytosol of NPs that were GR-alpha-negative to the binding assay. GR-beta was expressed in NPs and absent in the majority of NM. TGF-beta1 expression was higher in NPs than in NM.
CONCLUSIONS: GR-beta and TGF-beta1 might be involved in NP pathogenesis, but their role in modulating GC sensitivity is still unclear.
Authors:
S Aversa; C Ondolo; G Abbadessa; F Piccione; V Carriero; A Fulcheri; A Lauria; S De Francia; S Racca
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rhinology     Volume:  50     ISSN:  0300-0729     ISO Abbreviation:  Rhinology     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-29     Completed Date:  2013-03-01     Revised Date:  2013-11-07    
Medline Journal Info:
Nlm Unique ID:  0347242     Medline TA:  Rhinology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  427-35     Citation Subset:  IM    
Affiliation:
Otorhinolaryngology, Department of Clinical and Biological Sciences, University of Torino, San Luigi Hospital, Orbassano, Torino, Italy.
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MeSH Terms
Descriptor/Qualifier:
Drug Resistance
Electrophoresis, Polyacrylamide Gel
Endoscopy
Female
Glucocorticoids / therapeutic use
Humans
Male
Middle Aged
Nasal Polyps / drug therapy,  physiopathology*,  surgery
Prednisone / therapeutic use
Receptors, Glucocorticoid / physiology*
Transforming Growth Factor beta1 / physiology*
Chemical
Reg. No./Substance:
0/Glucocorticoids; 0/Receptors, Glucocorticoid; 0/Transforming Growth Factor beta1; 53-03-2/Prednisone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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