| Steroid hormone (hydrocortisone, oestradiol and testosterone) uptake, storage or induced synthesis in tetrahymena. | |
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MedLine Citation:
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PMID: 10873299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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After cyclodextrin-coated 10(-6) m steroid hormone treatment for 3 days (hormonal imprinting), Tetrahymena cells and their media were analysed by radioimmunoassay for the same hormone and for the presence of the other two. In the absence of hormone treatment, the cells contained no detectable levels of the three steroids. By 2 days in fresh medium following exposure of cells to a 72 h pretreatment of each specific hormone, correspondingly high quantities of hydrocortisone and oestradiol, but lesser quantities of testosterone, were found in both the media and the cells. One week after treatment only traces of hydrocortisone were found, exclusively within the cells themselves. Oestradiol was present in measurable quantities in both cells and media, whereas testosterone was only present in the medium. The presence of the other two hormones to the one used in the pretreatment were not usually present, except that when testosterone had been given, some oestradiol was also detected at 48 h, suggesting Tetrahymena has a functional cytochrome P(450)aromatase. |
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Authors:
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G Csaba; E Poteczin; T Fehér; P Kovács |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 22 ISSN: 1065-6995 ISO Abbreviation: Cell Biol. Int. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 2002-07-16 Completed Date: 2002-12-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 875-8 Citation Subset: IM |
Copyright Information:
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Copyright 1998 Academic Press. |
Affiliation:
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Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Culture Media / metabolism Cyclodextrins / pharmacology Cytochrome P-450 Enzyme System / drug effects, metabolism Dose-Response Relationship, Drug Drug Administration Schedule Estradiol / biosynthesis*, pharmacology Hydrocortisone / biosynthesis*, pharmacology Testosterone / biosynthesis*, pharmacology Tetrahymena / cytology, drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Cyclodextrins; 50-23-7/Hydrocortisone; 50-28-2/Estradiol; 58-22-0/Testosterone; 9035-51-2/Cytochrome P-450 Enzyme System |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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