Document Detail

Steroid Hormone Receptor Immunohistochemistry in Breast Cancer: Past, Present, and Future.
MedLine Citation:
PMID:  11348249     Owner:  NLM     Status:  Publisher    
This article reviews the development of steroid hormone receptor detection using the biochemical approach and the disadvantages inherent in these systems. The early history of methods for the in situ detection of receptors is related, culminating in the development of histologic immunoassays using monoclonal antibodies. Correlation of the latter with disease-free and overall survival and with clinical endocrine response are presented together with preliminary findings utilizing a new generation of antireceptor antibodies. Current problems with test scoring and interpretation are detailed with a section devoted to image analysis for assay quantification. Emphasis is placed on the fact that because so many different antibodies and tissue substrates have been employed in the past, these histologic receptor immunoassays cannot yet be considered to be fully validated. It is stressed that different laboratories must employ the same antibodies, substrates, detection systems and methods of scoring, and then correlate findings with measurable clinical end points. Only then can the assays be included in the brief list of approved breast cancer prognostic and predictive markers to eventually replace the routine biochemical methods of steroid receptor detection.
Louis P. Pertschuk; Constantine A. Axiotis
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Publication Detail:
Journal Detail:
Title:  The breast journal     Volume:  5     ISSN:  1524-4741     ISO Abbreviation:  Breast J     Publication Date:  1999 Jan 
Date Detail:
Created Date:  2001-May-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505539     Medline TA:  Breast J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  3-12     Citation Subset:  -    
Department of Pathology, State University of New York Health Science Center at Brooklyn and the Kings County Hospital Center, Brooklyn, New York.
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