| Sterilization of therapeutic immunoadsorbents with aqueous propylene oxide solution. | |
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MedLine Citation:
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PMID: 3921468 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Propylene oxide (PO), in the form of its aqueous solution, was evaluated as a chemical sterilant for therapeutic immunoadsorbent to be used in on-line plasmapheresis. Generally, PO is characterized as less toxic and less antimicrobial than ethylene oxide. PO was significant sporicidal to B. subtilis at 25 degrees C and 10(6) spores/ml were killed with 1% (V/V) PO solution after storage for 3 weeks when 75% to 80% of PO decomposed; the death-rate conformed to the kinetics of a first-order reaction although PO decomposed rapidly. Thus an apparent D values were estimated to be 13 to 16 days with 0.5% PO solution and 5 to 7 days with 1% PO. An immunoadsorbent consisting of Sepharose 4B immobilizing anti-human IgE antibody was used to study the effect of PO on adsorption characteristics. PO affected the IgE removal of the immunoadsorbents depending on its concentration ranging from 0% to 4%. The IgE-adsorptions were lowered by only 17% and 30% of the intact and glutaraldehyde-treated immunoadsorbents respectively after storage for 3 months in the 2% PO solution at 25 degrees C, and these numbers are considered to be acceptable for a clinical use. A 2% PO solution was chosen based on the results of the shelf life test of immunoadsorbents and the sporicidal test of PO. |
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Authors:
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H Sato; T Kidaka; M Hori |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The International journal of artificial organs Volume: 8 ISSN: 0391-3988 ISO Abbreviation: Int J Artif Organs Publication Date: 1985 Mar |
Date Detail:
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Created Date: 1985-06-07 Completed Date: 1985-06-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7802649 Medline TA: Int J Artif Organs Country: ITALY |
Other Details:
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Languages: eng Pagination: 109-14 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Antigen-Antibody Complex* Bacillus subtilis / drug effects Drug Stability Drug Storage Epoxy Compounds* / pharmacology Ethers, Cyclic* Humans Immunoglobulin E Immunosorbents* Plasmapheresis* Sterilization* |
| Chemical | |
Reg. No./Substance:
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0/Antigen-Antibody Complex; 0/Epoxy Compounds; 0/Ethers, Cyclic; 0/Immunosorbents; 37341-29-0/Immunoglobulin E; 75-56-9/propylene oxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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