Document Detail


Stereotactic transplantation of a dopamine-producing capsule into the striatum for treatment of Parkinson disease: a preclinical primate study.
MedLine Citation:
PMID:  12691415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECT: The PC12 cells are well known for their ability to secrete dopamine and levodopa. In multiple animal mode encapsulated PC12 cells have been shown to ameliorate parkinsonian symptoms when transplanted into the striatum; technique is expected to be effective clinically as well. The present study was performed using nonhuman primates to ensure that the transplantation of encapsulated PC12 cells is likely to be both safe and effective in human clinical trials. METHODS: Unencapsulated or encapsulated PC12 cells were implanted into the brains of Japanese monkeys (Macaca fuscata). Histological and immunocytochemical analyses were performed 1, 2, 4, and 8 weeks posttransplantation on the unencapsulated cells and 2, 4, and 8 weeks after transplantation on the encapsulated cells. The survival of the PC12 cells inside the capsule was determined by measuring the amounts of dopamine and levodopa released from the capsules a removal from the striatum. Magnetic resonance imaging was performed in both unencapsulated and encapsulated PC12 cell-grafted groups. Due to the immunological reaction of the host brain no unencapsulated PC12 cells remained in the grafted area 8 weeks after transplantation. On the contrary, encapsulated PC12 cells retrieved from the host brain continued to release dopamine and levodopa even 8 weeks after implantation. The host's reaction to the PC12-loaded capsule was much weaker than that to the unencapsulated PC12 cells. CONCLUSIONS: These results suggest that the transplantation of encapsulated PC12 cells could be a safe and effective treatment modality for Parkinson disease in human patients.
Authors:
Hideyuki Yoshida; Isao Date; Tetsuro Shingo; Kenjiro Fujiwara; Kazuki Kobayashi; Yasuyuki Miyoshi; Takashi Ohmoto
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurosurgery     Volume:  98     ISSN:  0022-3085     ISO Abbreviation:  J. Neurosurg.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-14     Completed Date:  2003-05-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0253357     Medline TA:  J Neurosurg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  874-81     Citation Subset:  AIM; IM    
Affiliation:
Department of Neurological Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Tissue Transplantation / instrumentation*
Chromatography, High Pressure Liquid
Corpus Striatum / metabolism,  pathology,  transplantation*
Dopamine / metabolism*
Immunohistochemistry
Levodopa / metabolism
Macaca
Magnetic Resonance Imaging
PC12 Cells / metabolism,  transplantation*
Parkinson Disease / metabolism,  pathology,  surgery*
Rats
Stereotaxic Techniques / instrumentation*
Chemical
Reg. No./Substance:
0/Levodopa

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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