Document Detail

Stereology of the lung.
MedLine Citation:
PMID:  23317906     Owner:  NLM     Status:  In-Data-Review    
Many scientific projects require a quantitative assessment of organ, tissue and cell (ultra)structure. Such quantitative (morphometric) data are essential to make statistically valid comparisons between experimental groups. The structures of interest are measured at different microscopic levels. However, measurements in microscopy pose two problems: 1) Only a small fraction of the whole biological system can be analyzed (sampling problem). 2) The analysis is performed on nearly two-dimensional (physical, optical or virtual) sections through the object although the aim is to obtain biologically meaningful three-dimensional data (3D vs 2D problem). These problems are solved by the application of unbiased sampling and measurement tools known as stereology. This chapter gives a brief introduction to the theory and practical application of stereology, using the lung as an example. Stereological tools needed to quantify volume, number and surface area are introduced and examples are given how to estimate total lung volume, volume of lung parenchyma, alveolar surface area and number of alveolar epithelial type II cells per lung.
Jan Philipp Schneider; Matthias Ochs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in cell biology     Volume:  113     ISSN:  0091-679X     ISO Abbreviation:  Methods Cell Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373334     Medline TA:  Methods Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  257-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
Institute of Functional and Applied Anatomy Hannover Medical School, Hannover, Germany.
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