Document Detail


Stereological study of postnatal testicular development in Blackbelly sheep.
MedLine Citation:
PMID:  17583781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective was to characterize testicular development in Blackbelly sheep, focusing primarily on Sertoli cell number. Lambs (n=43) were allotted into eight groups, and surgically castrated at 0, 3, 6, 9, 12, 15,18 or 21 weeks of age (n=4-6 lambs per group). Testes were fixed and paraffin-embedded, cross-sections (5 microm) were stained and evaluated with quantitative morphometry techniques. Testis weight increased at a greater rate between 9 and 15 weeks of age, which was associated with remarkable changes in testicular histology, including increases in tubular tissue volume, and tubule diameter and length. Spermatogenesis started in a period between 9 and 12 weeks, lumen and elongated spermatids were observed for the first time at 12 weeks (78% of the tubules) and 15 weeks (37% of the tubules), respectively. Total number of Sertoli cells (mean+/-S.E.M.) increased steadily from birth (531+/-76 x 10(6)) to 15 weeks (12,008+/-1722 x 10(6)), with no changes afterwards. Sertoli cell number per gram of testicular tissue decreased as lambs were older, with the most remarkable change occurring between Weeks 9 and 12. An early increase in serum LH was observed at 6 weeks of age, with testosterone (T) increasing at Weeks 12 and 21. In conclusion, Sertoli cells maintained the capacity of proliferating from birth to 15 weeks of age in Blackbelly sheep; furthermore, the period of accelerated testis growth was associated with increased serum T concentration and with important changes in testicular morphology, as a consequence of the beginning and establishment of spermatogenesis and Sertoli cell maturation.
Authors:
J Herrera-Alarcón; E Villagómez-Amezcua; E González-Padilla; H Jiménez-Severiano
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-20
Journal Detail:
Title:  Theriogenology     Volume:  68     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-07-30     Completed Date:  2007-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  582-91     Citation Subset:  IM    
Affiliation:
Facultad de Estudios Superiores Cuautitlán UNAM, Campus Ajuchitlán, Querétaro Qro, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight / physiology
Histocytochemistry / veterinary
Luteinizing Hormone / blood
Male
Organ Size / physiology
Random Allocation
Regression Analysis
Sertoli Cells / cytology,  physiology
Sheep / blood,  growth & development*
Spermatogenesis / physiology*
Testis / cytology,  growth & development*
Testosterone / blood
Thyroxine / blood
Triiodothyronine / blood
Chemical
Reg. No./Substance:
58-22-0/Testosterone; 6893-02-3/Triiodothyronine; 7488-70-2/Thyroxine; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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