Document Detail

Stereological methods: a new approach in the assessment of pulmonary emphysema.
MedLine Citation:
PMID:  8842025     Owner:  NLM     Status:  MEDLINE    
In order to develop a reliable and sensitive method for studying the development and progression of pulmonary emphysema, we compared stereological indices with the usual index for grade of emphysema, i.e., the mean linear intercept (Lm), in elastase-induced emphysema in mice. The Lm and stereological indices, including volumes of total lung tissue (V(lt)), airspaces (V(air)), and surface area of alveolar walls (S(alv)), were determined in 5-microns, H&E-stained, paraffin-embedded lung sections from elastase- (n = 7) or saline-treated (n = 8) mice. The indices were measured by point counting, using Cavalieri's principle (V(lt)) and V(air)) or by counting intersections of alveolar walls with test lines of a known length (S(alv) and Lm). Elastase treatment resulted in a significant increase of Lm and of V(air), both indicating airspace enlargement, and in a significant decrease of V(lt) and S(alv), indicating destruction of alveolar walls. Between each of the stereological indices and the Lm, significant correlations were found when all lungs were included, but not when the emphysematous lungs were considered separately. We conclude that stereological methods can be powerful morphometric tools for studying pulmonary emphysema development and progression, since they give information not only about the grade of airspace enlargement but also about the grade of destruction of alveolar walls. Based on this unique property, stereological methods also allow a distinction between pulmonary emphysema and unrelated conditions with dilatation of airspaces only.
B A Heemskerk-Gerritsen; J H Dijkman; A A Ten Have-Opbroek
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microscopy research and technique     Volume:  34     ISSN:  1059-910X     ISO Abbreviation:  Microsc. Res. Tech.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-12-19     Completed Date:  1996-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9203012     Medline TA:  Microsc Res Tech     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  556-62     Citation Subset:  IM    
Department of Pulmonology, University of Leiden, The Netherlands.
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MeSH Terms
Lung / pathology*
Pancreatic Elastase / toxicity
Pulmonary Emphysema / pathology*
Reg. No./Substance:
EC Elastase

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