Document Detail


Stepwise multiphoton activation fluorescence reveals a new method of melanin detection.
MedLine Citation:
PMID:  23291783     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The stepwise multiphoton activated fluorescence (SMPAF) of melanin, activated by a continuous-wave mode near infrared (NIR) laser, reveals a broad spectrum extending from the visible spectra to the NIR and has potential application for a low-cost, reliable method of detecting melanin. SMPAF images of melanin in mouse hair and skin are compared with conventional multiphoton fluorescence microscopy and confocal reflectance microscopy (CRM). By combining CRM with SMPAF, we can locate melanin reliably. However, we have the added benefit of eliminating background interference from other components inside mouse hair and skin. The melanin SMPAF signal from the mouse hair is a mixture of a two-photon process and a third-order process. The melanin SMPAF emission spectrum is activated by a 1505.9-nm laser light, and the resulting spectrum has a peak at 960 nm. The discovery of the emission peak may lead to a more energy-efficient method of background-free melanin detection with less photo-bleaching.
Authors:
Zhenhua Lai; Josef Kerimo; Yair Mega; Charles A Dimarzio
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  18     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-01-07     Completed Date:  2013-06-06     Revised Date:  2013-08-06    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  061225     Citation Subset:  IM    
Affiliation:
Northeastern University, Electrical and Computer Engineering Department, Boston, MA 02115, USA. lai.z@husky.neu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Hair / chemistry,  radiation effects
Melanins / metabolism*,  radiation effects
Mice
Microscopy, Fluorescence, Multiphoton / instrumentation,  methods*
Optical Phenomena
Photobleaching
Skin / metabolism*,  radiation effects
Spectroscopy, Near-Infrared / instrumentation,  methods
Chemical
Reg. No./Substance:
0/Melanins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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