Document Detail


Stepwise commitment of blast cell fates during the positional specification of the O and P cell lines in the leech embryo.
MedLine Citation:
PMID:  6500176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The o and p bandlets of the leech embryo are parallel columns of ectodermal blast cells which are identified by their relative positions, and which during normal embryogenesis follow distinct developmental pathways. A previous study showed that o blast cells are initially capable of following either the O or P pathway, and suggested that commitment to the O pathway depends upon interaction with the adjacent p bandlet. To better understand the nature and timing of this interaction we examined the fate of o blast cells whose p blast cell neighbors had been selectively ablated by photoexcitation of a fluorescent lineage tracer. If an o blast cell has not yet begun its secondary divisions, its normal commitment to the O pathway can be effectively prevented by ablation of the adjacent p bandlet. Comparing the outcome of progressively later lesions reveals that the progeny of the o blast cell become committed to the O pathway in a series of three discrete steps, and that these steps occur around the time of the first three blast cell divisions. Each of the three events affects a different subset of elements within the blast cell clone, and apparently commits those elements to either the O or P pathway depending upon the presence or absence of the other bandlet. These changes in blast cell fate are coextensive with the lesion along the bandlet's length, suggesting that the interaction of the two bandlets is localized to neighboring cells.
Authors:
M Shankland; D A Weisblat
Related Documents :
9822716 - Identification and characterization of a novel line of drosophila schneider s2 cells th...
11149916 - Wasp, the drosophila wiskott-aldrich syndrome gene homologue, is required for cell fate...
14667406 - Drosophila nonmuscle myosin ii promotes the asymmetric segregation of cell fate determi...
7956836 - Marbles mutants: uncoupling cell determination and nuclear migration in the developing ...
9042386 - Increased microbicidal activity of human monoblastoid cells upon long-term exposure to ...
2445886 - The mechanism of action of decay-accelerating factor (daf). daf inhibits the assembly o...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  106     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-01-23     Completed Date:  1985-01-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  326-42     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blastoderm / cytology*,  physiology
Cell Differentiation
Cell Line
Ectoderm / cytology
Embryo, Nonmammalian / cytology
Endoderm / cytology
Female
Leeches / embryology*
Neurons / cytology,  physiology
Grant Support
ID/Acronym/Agency:
5 F32 NS06814-02/NS/NINDS NIH HHS; HD17088/HD/NICHD NIH HHS; NS12818/NS/NINDS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Formation of pattern in regenerating tissue pieces of Hydra attenuata. III. The shaping of the body ...
Next Document:  Modulation of nonglobin mRNA levels in erythropoiesis.