| Stepwise arteriovenous fate acquisition during mammalian vasculogenesis. | |
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MedLine Citation:
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PMID: 21793101 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Arteriovenous (AV) differentiation is a critical step during blood vessel formation and stabilization. Defects in arterial or venous fate lead to inappropriate fusion of vessels, resulting in damaging arteriovenous shunts. While many studies have unraveled the molecular underpinnings that drive AV fate, surprisingly, the spatiotemporal emergence of arteries and veins in mammalian embryos remains unknown. Here, we examine artery and vein specification and differentiation during vasculogenesis. We show that the first intraembryonic vessels formed are arteries, which differentiate in a stepwise manner. By contrast, veins emerge later, progressively forming after embryonic turning. In addition, we demonstrate that hemodynamic flow is not required for arterial specification, but is required for maintenance of select arterial markers. Together, our results provide a first spatiotemporal analysis of mammalian AV cell fate establishment and anatomy, as well as a delineation of a molecular toolkit for analysis of arteries and veins during early vessel development. |
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Authors:
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Diana C Chong; Yeon Koo; Ke Xu; Stephen Fu; Ondine Cleaver |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-07-25 |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 240 ISSN: 1097-0177 ISO Abbreviation: Dev. Dyn. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-10-13 Completed Date: 2012-05-15 Revised Date: 2012-09-28 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 2153-65 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arteries / embryology* Cell Differentiation / genetics, physiology Embryo, Mammalian / cytology, metabolism Female Fluorescent Antibody Technique Hemodynamics / genetics, physiology In Situ Hybridization Intracellular Signaling Peptides and Proteins / genetics, metabolism Membrane Proteins / genetics, metabolism Mice Pregnancy Veins / embryology* |
| Grant Support | |
ID/Acronym/Agency:
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DK079862/DK/NIDDK NIH HHS; R01 DK079862-01/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DLL4 protein, mouse; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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