Document Detail


Stepwise arteriovenous fate acquisition during mammalian vasculogenesis.
MedLine Citation:
PMID:  21793101     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arteriovenous (AV) differentiation is a critical step during blood vessel formation and stabilization. Defects in arterial or venous fate lead to inappropriate fusion of vessels, resulting in damaging arteriovenous shunts. While many studies have unraveled the molecular underpinnings that drive AV fate, surprisingly, the spatiotemporal emergence of arteries and veins in mammalian embryos remains unknown. Here, we examine artery and vein specification and differentiation during vasculogenesis. We show that the first intraembryonic vessels formed are arteries, which differentiate in a stepwise manner. By contrast, veins emerge later, progressively forming after embryonic turning. In addition, we demonstrate that hemodynamic flow is not required for arterial specification, but is required for maintenance of select arterial markers. Together, our results provide a first spatiotemporal analysis of mammalian AV cell fate establishment and anatomy, as well as a delineation of a molecular toolkit for analysis of arteries and veins during early vessel development.
Authors:
Diana C Chong; Yeon Koo; Ke Xu; Stephen Fu; Ondine Cleaver
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-07-25
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  240     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-10-13     Completed Date:  2012-05-15     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2153-65     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arteries / embryology*
Cell Differentiation / genetics,  physiology
Embryo, Mammalian / cytology,  metabolism
Female
Fluorescent Antibody Technique
Hemodynamics / genetics,  physiology
In Situ Hybridization
Intracellular Signaling Peptides and Proteins / genetics,  metabolism
Membrane Proteins / genetics,  metabolism
Mice
Pregnancy
Veins / embryology*
Grant Support
ID/Acronym/Agency:
DK079862/DK/NIDDK NIH HHS; R01 DK079862-01/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/DLL4 protein, mouse; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Proteins
Comments/Corrections

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