| Stem-loop probe with universal reporter for sensing unlabeled nucleic acids. | |
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MedLine Citation:
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PMID: 17509514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this article, we present the design principles and application of a motif composed of a stem-loop probe (SP) hybridized to a fluorescently labeled universal reporter (UR) for sensing unlabeled nucleic acids. At room temperature, SP-UR is in the hairpin-closed form in which the fluorophore of UR is in proximity to the G bases of the hairpin, where consequently the fluorescent emission is quenched significantly. On hybridization with target, SP-UR is trapped in the hairpin-opened configuration in which the fluorophore and the G quenchers are apart. This turns off quenching, increases emission intensity, and signals the presence of target. Compared with the common approach that employs an oligonucleotide probe with a covalently linked fluorophore, the use of a fluorescently labeled universal reporter strand hybridized to an unlabeled stem-loop probe provides a more efficient approach to the fabrication of nucleic acid sensors and microarrays potentially useful for real-time analysis. |
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Authors:
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Suk-Wah Tam-Chang; Travis D Carson; Liming Huang; Nelson G Publicover; Kenneth W Hunter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-03-13 |
Journal Detail:
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Title: Analytical biochemistry Volume: 366 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-06-18 Completed Date: 2007-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 126-30 Citation Subset: IM |
Affiliation:
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Department of Chemistry, College of Science, University of Nevada, Reno, NV 89557, USA. tchang@unr.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fluorescent Dyes
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chemistry Models, Biological Molecular Structure Nucleic Acid Conformation Nucleic Acid Hybridization / methods* Nucleic Acids / chemistry*, genetics Oligonucleotide Probes / chemical synthesis, chemistry* Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Nucleic Acids; 0/Oligonucleotide Probes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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