Document Detail

Stem-like cells in bladder cancer cell lines with differential sensitivity to cisplatin.
MedLine Citation:
PMID:  22399585     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Recurrence is a common problem in bladder cancer; this has been attributed to cancer stem cells. In this study, we characterized potential cancer stem cell populations isolated from three cell lines that demonstrate different responses to cisplatin.
MATERIALS AND METHODS: The ALDEFLUOR® assay was used to isolate cells from TCCSUP, T24, and 5637 cell lines, and these cells were evaluated for their ability to form colonies, differentiate, migrate and invade.
RESULTS: The cell lines demonstrate a spectrum of aldehyde dehydrogenase high (ALDH(High)) populations that correlate with resistance to cisplatin. In the two resistant cell lines, T24 and 5637, the ALDH(High) cells demonstrate increased colony formation, migration, invasion, and ability to differentiate. The resistant T24 and 5637 cell lines may serve as models to investigate alternative therapies for bladder cancer.
Miranda J Sarachine Falso; Bruce A Buchholz; Ralph W Devere White
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Anticancer research     Volume:  32     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-08     Completed Date:  2012-05-08     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  733-8     Citation Subset:  IM    
The Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, CA, USA.
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MeSH Terms
Aldehyde Dehydrogenase / metabolism
Antineoplastic Agents / pharmacology*
Cisplatin / pharmacology*
Drug Screening Assays, Antitumor
Neoplastic Stem Cells / drug effects*,  enzymology,  pathology
Urinary Bladder Neoplasms / enzymology,  pathology*
Grant Support
5P41RR013461-14/RR/NCRR NIH HHS; 8 P41 GM103483-14/GM/NIGMS NIH HHS; P41 GM103483/GM/NIGMS NIH HHS; P41 RR013461/RR/NCRR NIH HHS
Reg. No./Substance:
0/Antineoplastic Agents; 15663-27-1/Cisplatin; EC Dehydrogenase

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