| Stem-like cells in bladder cancer cell lines with differential sensitivity to cisplatin. | |
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MedLine Citation:
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PMID: 22399585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Recurrence is a common problem in bladder cancer; this has been attributed to cancer stem cells. In this study, we characterized potential cancer stem cell populations isolated from three cell lines that demonstrate different responses to cisplatin. MATERIALS AND METHODS: The ALDEFLUOR® assay was used to isolate cells from TCCSUP, T24, and 5637 cell lines, and these cells were evaluated for their ability to form colonies, differentiate, migrate and invade. RESULTS: The cell lines demonstrate a spectrum of aldehyde dehydrogenase high (ALDH(High)) populations that correlate with resistance to cisplatin. In the two resistant cell lines, T24 and 5637, the ALDH(High) cells demonstrate increased colony formation, migration, invasion, and ability to differentiate. The resistant T24 and 5637 cell lines may serve as models to investigate alternative therapies for bladder cancer. |
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Authors:
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Miranda J Sarachine Falso; Bruce A Buchholz; Ralph W Devere White |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Anticancer research Volume: 32 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-03-08 Completed Date: 2012-05-08 Revised Date: 2013-05-01 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 733-8 Citation Subset: IM |
Affiliation:
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The Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, CA, USA. falso1@llnl.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aldehyde Dehydrogenase
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metabolism Antineoplastic Agents / pharmacology* Cisplatin / pharmacology* Drug Screening Assays, Antitumor Humans Neoplastic Stem Cells / drug effects*, enzymology, pathology Urinary Bladder Neoplasms / enzymology, pathology* |
| Grant Support | |
ID/Acronym/Agency:
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5P41RR013461-14/RR/NCRR NIH HHS; 8 P41 GM103483-14/GM/NIGMS NIH HHS; P41 GM103483/GM/NIGMS NIH HHS; P41 RR013461/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 15663-27-1/Cisplatin; EC 1.2.1.3/Aldehyde Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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