| Stem cell marker TRA-1-60 is expressed in foetal and adult kidney and upregulated in tubulo-interstitial disease. | |
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MedLine Citation:
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PMID: 20853169 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The kidney has an intrinsic ability to repair itself when injured. Epithelial cells of distal tubules may participate in regeneration. Stem cell marker, TRA-1-60 is linked to pluripotency in human embryonic stem cells and is lost upon differentiation. TRA-1-60 expression was mapped and quantified in serial sections of human foetal, adult and diseased kidneys. In 8- to 10-week human foetal kidney, the epitope was abundantly expressed on ureteric bud and structures derived therefrom including collecting duct epithelium. In adult kidney inner medulla/papilla, comparisons with reactivity to epithelial membrane antigen, aquaporin-2 and Tamm-Horsfall protein, confirmed extensive expression of TRA-1-60 in cells lining collecting ducts and thin limb of the loop of Henle, which may be significant since the papillae were proposed to harbour slow cycling cells involved in kidney homeostasis and repair. In the outer medulla and cortex there was rare, sporadic expression in tubular cells of the collecting ducts and nephron, with positive cells confined to the thin limb and thick ascending limb and distal convoluted tubules. Remarkably, in cortex displaying tubulo-interstitial injury, there was a dramatic increase in number of TRA-1-60 expressing individual cells and in small groups of cells in distal tubules. Dual staining showed that TRA-1-60 positive cells co-expressed Pax-2 and Ki-67, markers of tubular regeneration. Given the localization in foetal kidney and the distribution patterns in adults, it is tempting to speculate that TRA-1-60 may identify a population of cells contributing to repair of distal tubules in adult kidney. |
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Authors:
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Irina Fesenko; Danielle Franklin; Paul Garnett; Paul Bass; Sara Campbell; Michelle Hardyman; David Wilson; Neil Hanley; Jane Collins |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-19 |
Journal Detail:
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Title: Histochemistry and cell biology Volume: 134 ISSN: 1432-119X ISO Abbreviation: Histochem. Cell Biol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-14 Completed Date: 2011-03-21 Revised Date: 2012-04-26 |
Medline Journal Info:
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Nlm Unique ID: 9506663 Medline TA: Histochem Cell Biol Country: Germany |
Other Details:
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Languages: eng Pagination: 355-69 Citation Subset: IM |
Affiliation:
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Infection, Inflammation and Immunity Division, School of Medicine, University of Southampton, Southampton, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Antigens, Surface / metabolism* Biological Markers / metabolism Female Fetus / metabolism Fluorescent Antibody Technique Gene Expression Regulation, Developmental Humans Immunohistochemistry Ki-67 Antigen / metabolism Kidney / embryology*, metabolism* Kidney Diseases / metabolism, pathology Kidney Medulla / embryology, metabolism Kidney Tubules, Distal / embryology, metabolism Male Middle Aged Nephritis, Interstitial / metabolism*, pathology Nephrons / embryology, metabolism PAX2 Transcription Factor / metabolism Pluripotent Stem Cells Proteoglycans / metabolism* Regeneration Stem Cells / metabolism* Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
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088566//Wellcome Trust; BB/D014670/2//Biotechnology and Biological Sciences Research Council; //Medical Research Council; //Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Biological Markers; 0/Ki-67 Antigen; 0/PAX2 Transcription Factor; 0/PAX2 protein, human; 0/Proteoglycans; 0/TRA-1-60 antigen, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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