Document Detail


Stem cell characteristics of transplanted rat mammary clonogens.
MedLine Citation:
PMID:  11010819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rat mammary glands contain a subpopulation of clonogenic epithelial cells with large proliferation and differentiation potentials. When transplanted, the clonogens in monodispersed rat mammary epithelial cell suspensions give rise to either alveolar units (AUs) or ductal units (DUs) depending on the nature of the hormonal milieu in the graft recipient. Clonogens are also the primary cells of origin of mammary cancer following exposure to ionizing radiation or chemical carcinogens. Given the other stem cell characteristics of mammary clonogens, it would be expected that the primary AUs and DUs to which they give rise when grafted and hormonally stimulated (a) would be derived from the same clonogenic cell subpopulation, (b) would contain all of the functionally differentiated cell types of homologous parts of comparably stimulated mammary glands in situ, and (c) would also contain clonogen subpopulations capable when subtransplanted of giving rise to secondary AUs and DUs of similar cell composition. The current experiments were designed to test these expectations. The data are discussed in the context of results of previous studies with this and other experimental models. The results further support the conclusion that rat mammary clonogens are multipotent mammary stem cells.
Authors:
N D Kim; T D Oberley; J Yasukawa-Barnes; K H Clifton
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  260     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-13     Completed Date:  2000-11-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  146-59     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
Department of Human Oncology, University of Wisconsin Medical School, Madison, Wisconsin 53792, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caseins / metabolism
Cell Differentiation
Cell Division
Cell Transplantation
Clone Cells / cytology,  metabolism
Female
Flow Cytometry
Immunohistochemistry
Laminin / metabolism
Mammary Glands, Animal / cytology*,  metabolism
Microscopy, Electron
Rats
Rats, Inbred F344
Rats, Inbred WF
Stem Cells / cytology*,  metabolism
Transplantation, Autologous
Transplantation, Isogeneic
Grant Support
ID/Acronym/Agency:
P30CA14520/CA/NCI NIH HHS; R37CA13881/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Caseins; 0/Laminin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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