Document Detail


Stem cells in thoracic aortic aneurysms and dissections: potential contributors to aortic repair.
MedLine Citation:
PMID:  22440369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The hallmark of thoracic aortic aneurysms and dissections (TAAD) is progressive medial degeneration, which can result from excessive tissue destruction and insufficient repair. Although multipotent stem cells (SCs) are important in tissue repair, their role in TAAD is unknown. We sought to determine whether SCs are more abundant in TAAD tissue than in control tissues, and whether SCs within the diseased aortic wall differentiate into functionally relevant cell types.
METHODS: Using immunohistochemistry, we compared the abundance of STRO-1+ cells, c-kit+ cells, and CD34+ cells in aortic tissue from patients with descending thoracic aortic aneurysms (n=12), patients with chronic descending thoracic aortic dissections (n=18), and age-matched organ donors (n=5). Using double immunofluorescence staining, we evaluated SC differentiation into smooth muscle cells, fibroblasts, and macrophages.
RESULTS: All three cell types were significantly more abundant in the media and adventitia of TAAD tissues than in control tissues. We identified subsets of STRO-1+ cells, c-kit+ cells, and CD34+ cells that also expressed the smooth muscle cell marker SM22-α or fibroblast-specific protein-1, suggesting SC differentiation into smooth muscle cells or fibroblasts. Other STRO-1+ cells expressed the macrophage marker CD68, suggesting differentiation into inflammatory cells.
CONCLUSIONS: Stem cells are more abundant in TAAD tissue than in normal aortic tissue. Differentiation of SCs into smooth muscle cells, fibroblasts, and inflammatory cells within the diseased aortic wall suggests that SCs might be involved in both reparative and destructive remodeling processes in TAAD. Understanding the regulation of SC-mediated aortic remodeling will be a critical step toward designing strategies to promote aortic repair and prevent adverse remodeling.
Authors:
Ying H Shen; Xiaoqing Hu; Sili Zou; Darrell Wu; Joseph S Coselli; Scott A LeMaire
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-20
Journal Detail:
Title:  The Annals of thoracic surgery     Volume:  93     ISSN:  1552-6259     ISO Abbreviation:  Ann. Thorac. Surg.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-30     Completed Date:  2012-07-12     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  15030100R     Medline TA:  Ann Thorac Surg     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1524-33     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2012 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.
Affiliation:
Texas Heart Institute at St. Luke's Episcopal Hospital, and Division of Cardiothoracic Surgery, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Aged
Analysis of Variance
Aneurysm, Dissecting / pathology,  surgery*
Aorta, Thoracic / cytology
Aortic Aneurysm, Thoracic / pathology,  surgery*
Case-Control Studies
Cell Differentiation / physiology*
Cells, Cultured
Female
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Male
Middle Aged
Muscle, Smooth, Vascular / cytology*
Reference Values
Sensitivity and Specificity
Stem Cell Transplantation / methods*
Tissue Culture Techniques
Tissue and Organ Harvesting
Treatment Outcome
Grant Support
ID/Acronym/Agency:
P50 HL083794/HL/NHLBI NIH HHS; P50 HL083794/HL/NHLBI NIH HHS; R01 HL085341/HL/NHLBI NIH HHS; R01 HL085341/HL/NHLBI NIH HHS; T32 HL007676/HL/NHLBI NIH HHS; T32 HL007676/HL/NHLBI NIH HHS; UL1 RR024148/RR/NCRR NIH HHS
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