Document Detail


Steady surface modification of polydimethylsiloxane microchannel and its application in simultaneous analysis of homocysteine and glutathione in human serum.
MedLine Citation:
PMID:  17761187     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel polydimethylsiloxane (PDMS) surface modification method for microchip electrophoresis has been developed to make a stable and sufficient electroosmotic flow (EOF). Poly(l-glutamic acid) (PGA) which had ionizable carboxyl groups at a high pH-range was immobilized on the surface of microchannel fabricated with PDMS. The surface modification involved surface oxidation by plasma, the silanization of 3-aminopropyldimethylethoxysilane (APDMES) and immobilization of PGA via amide bond. The modified channel was extremely stable against consecutive electric power supply over 5h, and its long-term stability was demonstrated by the efficient separation of four amino acid derivatives reproducibly after a week. Additionally, homocysteine (Hcy), important risk factor of cardiovascular disease, osteoporosis and problems in pregnancy, was successfully measured in human serum in modified PDMS channel with the other thio amino acid simultaneously.
Authors:
Kyo Miyaki; Hu-Lie Zeng; Tatsuro Nakagama; Katsumi Uchiyama
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Publication Detail:
Type:  Journal Article     Date:  2007-08-17
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1166     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-10     Completed Date:  2007-10-31     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  201-6     Citation Subset:  IM    
Affiliation:
Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, 1-1, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
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MeSH Terms
Descriptor/Qualifier:
Dimethylpolysiloxanes / chemistry
Electroosmosis
Electrophoresis, Microchip / instrumentation,  methods*
Glutathione / blood*
Homocysteine / blood*
Humans
Hydrogen-Ion Concentration
Polyglutamic Acid / chemistry
Reproducibility of Results
Silicones / chemistry
Surface Properties
Chemical
Reg. No./Substance:
0/Dimethylpolysiloxanes; 0/Silicones; 25513-46-6/Polyglutamic Acid; 454-28-4/Homocysteine; 63148-62-9/baysilon; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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