| Steady surface modification of polydimethylsiloxane microchannel and its application in simultaneous analysis of homocysteine and glutathione in human serum. | |
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MedLine Citation:
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PMID: 17761187 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel polydimethylsiloxane (PDMS) surface modification method for microchip electrophoresis has been developed to make a stable and sufficient electroosmotic flow (EOF). Poly(l-glutamic acid) (PGA) which had ionizable carboxyl groups at a high pH-range was immobilized on the surface of microchannel fabricated with PDMS. The surface modification involved surface oxidation by plasma, the silanization of 3-aminopropyldimethylethoxysilane (APDMES) and immobilization of PGA via amide bond. The modified channel was extremely stable against consecutive electric power supply over 5h, and its long-term stability was demonstrated by the efficient separation of four amino acid derivatives reproducibly after a week. Additionally, homocysteine (Hcy), important risk factor of cardiovascular disease, osteoporosis and problems in pregnancy, was successfully measured in human serum in modified PDMS channel with the other thio amino acid simultaneously. |
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Authors:
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Kyo Miyaki; Hu-Lie Zeng; Tatsuro Nakagama; Katsumi Uchiyama |
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Publication Detail:
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Type: Journal Article Date: 2007-08-17 |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1166 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2007 Sep |
Date Detail:
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Created Date: 2007-09-10 Completed Date: 2007-10-31 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 201-6 Citation Subset: IM |
Affiliation:
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Department of Applied Chemistry, Graduate School of Engineering, Tokyo Metropolitan University, 1-1, Minamiohsawa, Hachioji, Tokyo 192-0397, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Dimethylpolysiloxanes
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chemistry Electroosmosis Electrophoresis, Microchip / instrumentation, methods* Glutathione / blood* Homocysteine / blood* Humans Hydrogen-Ion Concentration Polyglutamic Acid / chemistry Reproducibility of Results Silicones / chemistry Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Dimethylpolysiloxanes; 0/Silicones; 25513-46-6/Polyglutamic Acid; 454-28-4/Homocysteine; 63148-62-9/baysilon; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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