| Steady state and time-resolved fluorescence properties of metastatic and non-metastatic malignant cells from different species. | |
| | |
MedLine Citation:
|
PMID: 8583278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Steady state and time-resolved fluorescence spectroscopy were employed to study the fluorescence from non-metastatic, metastatic and non-tumorigenic cell lines from different species. Excitations at 310 nm and 350 nm were used to monitor tryptophan and reduced nicotinamide adenine dinucleotide (NADH) fluorescence respectively. Subtle and consistent differences were observed between different categories of cell lines. It was found that the tryptophan to NADH fluorescence intensity ratio is higher in metastatic cell lines than in non-metastatic and normal cell lines. The fluorescence decay of the tryptophan residue in different cell lines was best described by triple exponential kinetics, whereas the NADH fluorescence decay was best described by mainly double and, in some cases, triple exponential kinetics. The average fluorescence lifetimes for tryptophan were in the range 2.5-3.7 ns. The average lifetime of NADH was lower (by a factor of approximately three) in metastatic cells than in non-metastatic cells and this finding is consistent for cell lines from different origins (rat or human). Correcting the fluorescence intensity for the average fluorescence lifetime of each species and for the volume of each cell line, it was shown that the concentrations of tryptophan and NADH are consistently higher in malignant metastatic cancer cells than in non-metastatic cells. |
| | |
Authors:
|
A Pradhan; P Pal; G Durocher; L Villeneuve; A Balassy; F Babai; L Gaboury; L Blanchard |
Related Documents
:
|
18327938 - Small-molecule fluorophores to detect cell-state switching in the context of high-throu... 1294288 - Changes in retinal pigment epithelial cell autofluorescence and protein expression asso... 21518048 - Classification and functions of enteroendocrine cells of the lower gastrointestinal tract. 2375398 - Spatial heterogeneity in striated muscle arteriolar tone, cell flow, and capillarity. 4394388 - Tyrosine transaminase induction by dexamethasone in a new rat liver cell line. 10213158 - Brain-derived and glial cell line-derived neurotrophic factors protect a catecholaminer... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of photochemistry and photobiology. B, Biology Volume: 31 ISSN: 1011-1344 ISO Abbreviation: J. Photochem. Photobiol. B, Biol. Publication Date: 1995 Dec |
Date Detail:
|
Created Date: 1996-03-20 Completed Date: 1996-03-20 Revised Date: 2007-07-23 |
Medline Journal Info:
|
Nlm Unique ID: 8804966 Medline TA: J Photochem Photobiol B Country: SWITZERLAND |
Other Details:
|
Languages: eng Pagination: 101-12 Citation Subset: IM |
Affiliation:
|
Département de Chimie, Université de Montréal, Que, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line Humans NAD / analysis Neoplasm Metastasis* Neoplasms / chemistry* Rats Species Specificity Spectrometry, Fluorescence* / methods Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
53-84-9/NAD |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Special needs of preterm infants in the management of pain and discomfort.
Next Document: Evidence for UV-B-induced DNA degradation in Euglena gracilis mediated by activation of metal-depend...