Document Detail


Steady-state levels of mitochondrial messenger RNA species characterize a predominant pathway culminating in apoptosis and shedding of HT29 human colonic carcinoma cells.
MedLine Citation:
PMID:  8788038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A differentiated human colonic epithelial cell has undergone relatively stable molecular, biochemical, and cellular alterations resulting in the acquisition of structures, activities, and functions that characterize it as one of at least three mature phenotypes: a columnar absorptive, secretory, or enteroendocrine cell. We have shown previously that induction of HT29 cells with the short-chain fatty acid sodium butyrate elevates alkaline phosphatase activity, a marker of the absorptive cell phenotype, and increases mitochondrial gene expression. Furthermore, this induction is accompanied by subsequent apoptosis and cell shedding. In this report, we have investigated the effects of forskolin, a potent inducer of the MUC2 gene in HT29 cells, a marker of the secretory phenotype, and have shown that neither apoptosis nor mitochondrial gene expression are significantly stimulated. Thus, differentiation along the secretory cell lineage may not play a major role in apoptosis of colonic epithelial cells. Moreover, we have also investigated two polar solvents, DMSO and dimethylformamide, which have been reported to induce a more differentiated, but as yet not well characterized, phenotype in colonic carcinoma cells in culture. Although neither polar solvent induces alkaline phosphatase expression or MUC2 expression, both induce significant apoptosis, again associated with significant elevation of mitochondrial gene expression. Thus, elevation of mitochondrial gene expression appears to be an important pathway in the induction of apoptosis in colonic epithelial cells in culture, whether or not markers characteristic of differentiation along either the absorptive or secretory cell lineage are induced.
Authors:
B G Heerdt; M A Houston; J J Rediske; L H Augenlicht
Related Documents :
17110788 - Tsa-induced cell death in prostate cancer cell lines is caspase-2 dependent and involve...
18097588 - Differential involvement of mitochondria during ursolic acid-induced apoptotic process ...
10991668 - Mitochondrial dysfunction--a pathogenetic factor in parkinson's disease.
8833408 - Rotenone, a mitochondrial nadh dehydrogenase inhibitor, induces cell surface expression...
1090478 - Gastrin cell hyperplasia in rats with chronic antral stimulation.
22847388 - A faster, high resolution, mtpa-gfp-based mitochondrial fusion assay acquiring kinetic ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  7     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-10-31     Completed Date:  1996-10-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  101-06     Citation Subset:  IM    
Affiliation:
Department of Oncology, Albert Einstein Cancer Center, Bronx, New York 10467, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Apoptosis / physiology
Cell Differentiation / drug effects
DNA, Neoplasm / drug effects
Dimethyl Sulfoxide / pharmacology
Dimethylformamide / pharmacology
Fatty Acids, Volatile / pharmacology
Flow Cytometry
Forskolin / pharmacology
Gene Expression Regulation, Neoplastic / drug effects,  physiology
HT29 Cells / cytology*,  physiology
Humans
Mitochondria / genetics,  metabolism*
Mucins / genetics
Propidium
RNA / metabolism*
RNA, Messenger / metabolism
Grant Support
ID/Acronym/Agency:
P0 CA1330/CA/NCI NIH HHS; R01 CA56858/CA/NCI NIH HHS; R29 CA59932/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Fatty Acids, Volatile; 0/Mucins; 0/RNA, Messenger; 0/RNA, mitochondrial; 36015-30-2/Propidium; 63231-63-0/RNA; 66428-89-5/Forskolin; 67-68-5/Dimethyl Sulfoxide; 68-12-2/Dimethylformamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Overexpression of poly(ADP-ribose) polymerase promotes cell cycle arrest and inhibits neutrophilic d...
Next Document:  Distinct patterns of Fas cell surface expression during development of T- or B-lymphocyte lineages i...