Document Detail

Steady-state levels of aquaporin 1 mRNA expression are increased in idiopathic polyhydramnios.
MedLine Citation:
PMID:  16522429     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Polyhydramnios is a condition associated with significant perinatal morbidity. While the exact pathophysiology of this condition is unknown in the absence of obvious anatomic or organic etiologies, impaired intramembranous water transport has been shown. Previous studies from our laboratory have shown that the water channel aquaporin 1 (AQP1) is expressed in human fetal membranes from term pregnancies with normal amniotic fluid (AF) volume. Therefore, we hypothesized that in pregnancies with idiopathic polyhydramnios, AQP1 expression might be reduced in fetal membranes from pregnancies with this AF volume disorder. STUDY DESIGN: Placentas were collected from women at term (37-40 weeks) who presented with either polyhydramnios (amniotic fluid index [AFI] >24.0 cm) or normal AF volume (AFI 5.0-23.9 cm). Immediately after delivery, the membranes (amnion and chorion) directly overlying the placenta and the free-floating reflected membranes were sampled (total of 4 samples from each placenta). RNA was isolated from each sample and expression was quantified using real-time reverse transcriptase polymerase chain reaction (PCR) and relative quantification of gene expression. RESULTS: Relative to pregnancies with normal AF volume, there was an increase in expression of the water channel AQP1 in all regions of the fetal membranes. The greatest increase (33-fold) was seen in the reflected amnion. CONCLUSION: AQP1 expression is increased in polyhydramnios. This finding suggests that alterations in AQP1 expression may be a compensatory response to and not a cause of idiopathic polyhydramnios. We speculate that therapies focused on regulating AQP1 expression may be useful for treating this condition.
Stephanie E Mann; Natalia Dvorak; Heather Gilbert; Robert N Taylor
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  194     ISSN:  1097-6868     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-08     Completed Date:  2006-05-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  884-7     Citation Subset:  AIM; IM    
Phoenix Integrated Residency in Obstetrics and Gynecology, Maricopa Medical Center, Phoenix, AZ 85008, USA.
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MeSH Terms
Aquaporin 1 / genetics*
Extraembryonic Membranes / chemistry
Placenta / chemistry
Polyhydramnios / genetics*,  metabolism
RNA, Messenger / analysis,  biosynthesis*
Grant Support
Reg. No./Substance:
0/RNA, Messenger; 146410-94-8/Aquaporin 1

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