Document Detail


Staurosporine modulates radiosensitivity and radiation-induced apoptosis in U937 cells.
MedLine Citation:
PMID:  16546908     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The present study aims at investigating the involvement of several genes in the cell cycle distribution and apoptosis in U937 cells, a cell line lacking functional p53 protein, after combined treatment with staurosporine and irradiation. MATERIALS AND METHODS: Using a DNA fragmentation assay, flow cytometry and western blot analysis, the molecular basis for the effects of staurosporine in combination with the irradiation of leukemia cells was investigated. RESULTS: Our results indicated that combined treatment led to an increased apoptotic cell death in U937 cells, which is correlated with the phosphorylation of the V-Jun sarcoma virus 17 oncogene homolog (c-JUN) NH(2)-terminal kinase protein (JNK), the activation of caspases, the increase in B cell leukemia/lymphoma 2 (Bcl-2) associated X protein (Bax), the decrease in Bcl xL protein (Bcl-XL) levels, the loss of mitochondria membrane potential and the release of cytochrome c. CONCLUSIONS: Abrogation of the G2 checkpoint should be an effective strategy against p53-deficient leukemia cells to irradiation-induced cell killing.
Authors:
How-Ran Guo; Chia-Hsin Chen; Sheng-Yow Ho; Yuan-Soon Ho; Rong-Jane Chen; Ying-Jan Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  82     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-03-20     Completed Date:  2006-06-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  97-109     Citation Subset:  IM; S    
Affiliation:
Department of Environmental and Occupational Health, National Cheng Kung University, Medical College, Tainan, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*,  radiation effects*
Cell Cycle / drug effects*,  radiation effects*
Cell Cycle Proteins / metabolism*
Humans
Radiation Tolerance / drug effects
Radiation-Sensitizing Agents / administration & dosage*
Staurosporine / administration & dosage*
U937 Cells
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Radiation-Sensitizing Agents; 62996-74-1/Staurosporine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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