Document Detail


Staurosporine induces apoptosis in human papillomavirus positive oral cancer cells at G2/M phase by disrupting mitochondrial membrane potential and modulation of cell cytoskeleton.
MedLine Citation:
PMID:  19502099     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our study demonstrates that staurosporine (STS), a protein kinase inhibitor from Streptomyces sp., induces apoptosis in human papillomavirus positive oral carcinoma cells (KB) in a dose dependent manner. Growth inhibition studies revealed an IC(50) value of approximately 100 nM. STS induced marked nuclear fragmentation and inter-nucleosomal cleavage compared to untreated cells. It also caused dose dependent disruption of mitochondrial membrane potential and activation of caspase-3, indicating involvement of mitochondria-mediated cell death signaling in KB cell apoptosis. We found time-dependent arrest of the KB cells at G2/M phase of cell cycle. Using fluorescence microscopy, we have further shown that STS treatment disrupts microtubules and reorganizes F-actin after 6h exposure. Taken together, our results suggest that STS induces mitochondria-mediated KB cell apoptosis at G2/M phase by altering cell cytoskeletal network.
Authors:
Jayanta Sarkar; Neetu Singh; Sanjeev Meena; Sudhir Sinha
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-06
Journal Detail:
Title:  Oral oncology     Volume:  45     ISSN:  1879-0593     ISO Abbreviation:  Oral Oncol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-20     Completed Date:  2011-03-24     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  974-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Apoptosis / drug effects*
Caspase 3 / metabolism
Cell Cycle / drug effects
Cell Line, Tumor / drug effects
Enzyme Inhibitors / pharmacology*
G2 Phase / drug effects
Humans
Membrane Potential, Mitochondrial / drug effects*
Microscopy, Fluorescence
Microtubules / drug effects*
Mouth Neoplasms / metabolism*,  virology
Papillomaviridae*
Staurosporine / pharmacology*
Chemical
Reg. No./Substance:
0/Actins; 0/Enzyme Inhibitors; EC 3.4.22.-/Caspase 3; H88EPA0A3N/Staurosporine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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