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Status of proinflammatory and anti-inflammatory cytokines in different brain regions of a rat model of Japanese encephalitis.
MedLine Citation:
PMID:  22207393     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVES: This study evaluated cytokines and chemokine in different regions of the rat brain at different time points following Japanese encephalitis virus (JEV) infection. DESIGN: Twelve-day-old Wistar rats were infected by intracerebral inoculation of 3 × 10(6) plaque-forming units of JEV 78668A strain. Expression of cytokines and chemokine was assayed using cytokine bead array in different regions of the brain at 3, 6, 10 and 20 days post-inoculation (dpi). Pathological changes including immunohistochemistry for JEV antigen were observed. RESULTS: The cytokine levels were increased in the acute stage and declined on follow-up. In the acute stage (6 dpi), the levels of TNF-α, IFN-γ, IL-6 and IL-10 were maximum in the cortex, whereas the level of IL-4 was maximum in the striatum. Lower levels of these cytokines were observed in mid-brain and thalamus compared to other regions studied. Maximum expression of JEV antigen and histopathological changes as well as cytokines and chemokine were observed at 6 dpi in all the brain regions studied, but declined thereafter. CONCLUSION: This experimental study revealed maximum expression of cytokines and chemokine at 6 dpi of JEV infection which corresponded with histopathological changes in different brain regions.
Authors:
Ruchi Srivastava; Jayantee Kalita; Mohammad Yahiya Khan; Usha Kant Misra
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-30
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  -     ISSN:  1420-908X     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Neurology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareily Road, Lucknow, 226014, India.
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