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Status of mitochondria in living human fibroblasts during growth and senescence in vitro: use of the laser dye rhodamine 123.
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MedLine Citation:
PMID:  7309788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhodamine 123, a fluorescent laser dye that is selectively taken up into mitochondria of living cells, was used to examine mitochondrial morphology in early-passage (young), late-passage (old), and progeric human fibroblasts. Mitochondria were readily visualized in all cell types during growth (mid-log) and confluent stages. In all cell strains at confluence, mitochondria became shorter, more randomly aligned, and developed a higher proportion of bead-like forms. Treatment of cells for six days with Tevenel, a chloramphenicol analog that inhibits mitochondrial protein synthesis, brought about a marked depletion of mitochondria and a diffuse background fluorescence. Cyanide produced a rapid release of preloaded mitochondrial fluorescence followed by detachment and killing of cells. Colcemid caused a random coiling and fragmentation of mitochondria particularly in the confluent stage. No gross differences were discernible in mitochondria of the three cell strains in mid-log and confluent states or after these treatments. Butanol-extractable fluorescence after loading with rhodamine 123 was lower in all cell strains in confluent compared to mid-log stages. At confluence all three cell strains had similar rhodamine contents at zero-time and after washout up to 24 h. At the mid-log stage, young cells contained more rhodamine initially and lost it more rapidly than old or progeria cells, in that order. The data indicate no gross derangement in the morphology or number of mitochondria in old and progeria fibroblasts but there is a reduction of protonmotive force evident in these cells at the mid-log stage that may be growth limiting.
Authors:
S Goldstein; L B Korczack
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  91     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1982-02-12     Completed Date:  1982-02-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  392-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Cell Survival*
Chloramphenicol / analogs & derivatives,  pharmacology
Cyanides / pharmacology
Demecolcine / pharmacology
Fibroblasts / ultrastructure*
Humans
Mitochondria / drug effects,  ultrastructure*
Progeria / pathology*
Rhodamines
Chemical
Reg. No./Substance:
0/Cyanides; 0/Rhodamines; 4302-95-8/tevenel; 477-30-5/Demecolcine; 56-75-7/Chloramphenicol
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 11 Year: 1981
Volume: 91 Issue: 2
First Page: 392 Last Page: 398
ID: 2111971
Publisher Id: 82076000
PubMed Id: 7309788

Status of mitochondria in living human fibroblasts during growth and senescence in vitro: use of the laser dye rhodamine 123


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