Document Detail


Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y.enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus.
MedLine Citation:
PMID:  8895564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Yersinia Yop virulon is an anti-host system made up of four elements: (i) a type III secretion system called Ysc; (ii) a system designed to deliver bacterial proteins into eukaryotic target cells (YopB, YopD); (iii) a control element (YopN); and (iv) a set of intracellularly delivered proteins designed to disarm these cells or disrupt their communications (YopE, YopH and possibly others). YopM, another Yop protein, binds thrombin and is thus presumed to act as an extracellular effector. Here, we analyzed YopM from Y.enterocolitica and we wondered whether it could also be delivered inside eukaryotic cells. To answer this question we applied the Yop-Cya reporter strategy. Hybrids made of 141 or 100 N-terminal residues of YopM fused to Cya were delivered inside PU5-1.8 macrophages by recombinant Y.enterocolitica strains. YopB and YopD were required as translocators. Leakage of the reporters into the macrophage culture supernatant during the bacterial infection increased strongly when YopN was missing, showing that YopN is involved in the control of delivery of YopM inside eukaryotic cells. YopN itself was not delivered into the macrophages. In conclusion, YopM is translocated inside the eukaryotic cells and its physiopathological role should be revised or completed.
Authors:
A Boland; M P Sory; M Iriarte; C Kerbourch; P Wattiau; G R Cornelis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  15     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-12-16     Completed Date:  1996-12-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  5191-201     Citation Subset:  IM    
Affiliation:
Microbial Pathogenesis Unit, International Institute of Cellular and Molecular Pathology and Faculté de Médecine, Université Catholique de Louvain, Brussels, Belgium.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Z69926
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase
Adenylate Cyclase Toxin
Bacterial Outer Membrane Proteins / genetics,  metabolism*,  physiology*
Bacterial Proteins / genetics,  metabolism*
Biological Transport
Cytosol / metabolism
Genes, Bacterial / genetics
Genes, Reporter / genetics
Hela Cells / microbiology
Humans
Macrophages / metabolism*,  microbiology
Membrane Proteins*
Molecular Sequence Data
Mutation
Protein Precursors / genetics
Recombinant Fusion Proteins
Regulon / genetics
Yersinia enterocolitica / metabolism,  pathogenicity*
Chemical
Reg. No./Substance:
0/Adenylate Cyclase Toxin; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Membrane Proteins; 0/Protein Precursors; 0/Recombinant Fusion Proteins; 0/YopB protein, Yersinia; 0/YopD protein, Yersinia; 124893-29-4/yopM protein, Yersinia; 138341-76-1/YopN protein, Yersinia; EC 4.6.1.1/Adenylate Cyclase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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