| Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51. | |
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MedLine Citation:
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PMID: 18051763 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4 x 7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10x to 105x, which is ten times higher than the non-optimized rich medium. |
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Authors:
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Sanket Joshi; Sanjay Yadav; Anuradha Nerurkar; Anjana J Desai |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of microbiology and biotechnology Volume: 17 ISSN: 1017-7825 ISO Abbreviation: J. Microbiol. Biotechnol. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-01-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9431852 Medline TA: J Microbiol Biotechnol Country: Korea (South) |
Other Details:
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Languages: eng Pagination: 313-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Biotechnology Centre, Maharaja Sayajirao University of Baroda, Vadodara 390002, Gujarat, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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metabolism* Culture Media Micelles Surface-Active Agents / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Micelles; 0/Surface-Active Agents |
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