| Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate. | |
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MedLine Citation:
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PMID: 17043817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The nutritional requirements for acetoin production by Bacillus subtilis CICC 10025 were optimized statistically in shake flask experiments using indigenous agroindustrial by-products. The medium components considered for initial screening in a Plackett-Burman design comprised a-molasses (molasses submitted to acidification pretreatment), soybean meal hydrolysate (SMH), KH(2)PO(4).3H(2)O, sodium acetate, MgSO(4).7H(2)O, FeCl(2), and MnCl(2), in which the first two were identified as significantly (at the 99% significant level) influencing acetoin production. Response surface methodology was applied to determine the mutual interactions between these two components and optimal levels for acetoin production. In flask fermentations, 37.9 g l(-1) acetoin was repeatedly achieved using the optimized concentrations of a-molasses and SMH [22.0% (v/v) and 27.8% (v/v), respectively]. a-Molasses and SMH were demonstrated to be more productive than pure sucrose and yeast extract plus peptone, respectively, in acetoin fermentation. In a 5-l fermenter, 35.4 g l(-1) of acetoin could be obtained after 56.4 h of cultivation. To our knowledge, these results, i.e., acetoin yields in flask or fermenter fermentations, were new records on acetoin fermentation by B. subtilis. |
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Authors:
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Z J Xiao; P H Liu; J Y Qin; P Xu |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-17 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 74 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-01-26 Completed Date: 2007-03-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 61-8 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetoin
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metabolism* Bacillus subtilis / growth & development*, metabolism Biotechnology / methods* Culture Media / chemistry* Fermentation Models, Biological Molasses* Protein Hydrolysates / metabolism* Research Design Soybeans / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Protein Hydrolysates; 513-86-0/Acetoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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