Document Detail


Statistical optimization of medium components for enhanced acetoin production from molasses and soybean meal hydrolysate.
MedLine Citation:
PMID:  17043817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nutritional requirements for acetoin production by Bacillus subtilis CICC 10025 were optimized statistically in shake flask experiments using indigenous agroindustrial by-products. The medium components considered for initial screening in a Plackett-Burman design comprised a-molasses (molasses submitted to acidification pretreatment), soybean meal hydrolysate (SMH), KH(2)PO(4).3H(2)O, sodium acetate, MgSO(4).7H(2)O, FeCl(2), and MnCl(2), in which the first two were identified as significantly (at the 99% significant level) influencing acetoin production. Response surface methodology was applied to determine the mutual interactions between these two components and optimal levels for acetoin production. In flask fermentations, 37.9 g l(-1) acetoin was repeatedly achieved using the optimized concentrations of a-molasses and SMH [22.0% (v/v) and 27.8% (v/v), respectively]. a-Molasses and SMH were demonstrated to be more productive than pure sucrose and yeast extract plus peptone, respectively, in acetoin fermentation. In a 5-l fermenter, 35.4 g l(-1) of acetoin could be obtained after 56.4 h of cultivation. To our knowledge, these results, i.e., acetoin yields in flask or fermenter fermentations, were new records on acetoin fermentation by B. subtilis.
Authors:
Z J Xiao; P H Liu; J Y Qin; P Xu
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-10-17
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  74     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-26     Completed Date:  2007-03-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  61-8     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Acetoin / metabolism*
Bacillus subtilis / growth & development*,  metabolism
Biotechnology / methods*
Culture Media / chemistry*
Fermentation
Models, Biological
Molasses*
Protein Hydrolysates / metabolism*
Research Design
Soybeans / metabolism*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Protein Hydrolysates; 513-86-0/Acetoin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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