Document Detail

Stationary-phase mutations in proofreading exonuclease-deficient strains of the yeast Saccharomyces cerevisiae.
MedLine Citation:
PMID:  11361348     Owner:  NLM     Status:  MEDLINE    
In order to understand the role of yeast polymerases in spontaneous mutagenesis in non-growing cells we have studied the effects of mutations that impair the 3'--> 5' exonuclease function of polymerases delta (pol3-01) and epsilon (pol2-4) on the spontaneous reversion frequency of the frameshift mutation his7-2 in cells starved for histidine. We showed that for each exonuclease-deficient mutant the rate of reversion per viable cell per day observed in stationary-phase cells remained constant up to the 9th day of starvation (while the number of viable cells dropped), and was very similar to that observed in the same mutants during the growth phase. These data suggest that both DNA polymerases are involved in the control of mutability in non-growing cells.
N Babudri; Y I Pavlov; N Matmati; C Ludovisi; A Achilli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular genetics and genomics : MGG     Volume:  265     ISSN:  1617-4615     ISO Abbreviation:  Mol. Genet. Genomics     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-21     Completed Date:  2001-06-21     Revised Date:  2009-07-24    
Medline Journal Info:
Nlm Unique ID:  101093320     Medline TA:  Mol Genet Genomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  362-6     Citation Subset:  IM    
Department of Cellular Molecular Biology, University of Perugia, Italy.
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MeSH Terms
DNA Polymerase II / genetics,  physiology*
DNA Polymerase III / genetics,  physiology*
Exodeoxyribonuclease V
Exodeoxyribonucleases / genetics,  physiology*
Fungal Proteins / genetics,  physiology*
Saccharomyces cerevisiae / enzymology*,  genetics,  growth & development
Saccharomyces cerevisiae Proteins*
Reg. No./Substance:
0/CDC2 protein, S cerevisiae; 0/Fungal Proteins; 0/Saccharomyces cerevisiae Proteins; EC 2.7.7.-/DNA Polymerase II; EC 2.7.7.-/DNA Polymerase III; EC 3.1.-/Exodeoxyribonucleases; EC V

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