| Statin, a nonproliferation-specific protein, is associated with the nuclear envelope and is heterogeneously distributed in cells leaving quiescent state. | |
| | |
MedLine Citation:
|
PMID: 2674158 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Statin, a protein of 57,000 daltons, is present primarily in the nuclei of nonproliferating cells of terminally differentiated tissues or of in vitro aged fibroblast cultures. In young growing cells, the protein can be induced to appear in the nuclei once the cell-cycle traverse is blocked by various tissue culture manipulations, such as serum starvation; this expression, however, can be rapidly removed by addition of serum. The disappearance of statin in cells leaving the quiescent state is not uniform along the periphery of the nucleus; it can be distributed in various patterns, such as caps, nodules, patches, or irregular granules. This unusual distribution seems to suggest that preferential sites exist at the region of the nuclear envelope where statin presence may residually remain. The concentration of statin at the nuclear envelope region in cells at G0-quiescent phase is confirmed by the intense staining of fluorescent antibody at the periphery of isolated rat liver nuclei. Further examination of the isolated nuclei reveals that the protein is associated with the lamina compartment of the nuclear envelope; this is evidenced by the results of immunoblotting experiments showing statin presence in the fraction enriched for lamins A-C. Immunogold labelling studies show that the protein is located in the general area of the nuclear envelope. These results suggest that statin in G0-quiescent cells is located predominantly at the nuclear envelope region and that in this vicinity there may exist geometrically sites of statin concentration as evidenced by the heterogeneous distribution in those cells experiencing the departure from the quiescent state. |
| | |
Authors:
|
E Wang |
Related Documents
:
|
7743898 - Cell membrane-dependent chromatin condensation. 21114538 - Apr-246 exhibits anti-leukemic activity and synergism with conventional chemotherapeuti... 16450788 - A systematic approach to identifying urothelial cells likely to be polysomic by fluores... 3127728 - Translational control of insp3-induced chromatin condensation during the early cell cyc... 9791108 - Gigantism in a bacterium, epulopiscium fishelsoni, correlates with complex patterns in ... 11286328 - Non-invasive measurement of cell membrane associated proton gradients by ion-sensitive ... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of cellular physiology Volume: 140 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 1989 Sep |
Date Detail:
|
Created Date: 1989-10-25 Completed Date: 1989-10-25 Revised Date: 2010-09-14 |
Medline Journal Info:
|
Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 418-26 Citation Subset: IM |
Affiliation:
|
Bloomfield Centre for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montréal, Québec, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Compartmentation Cell Cycle* Cell Cycle Proteins Cell Line Fluorescent Antibody Technique Humans Immunohistochemistry Molecular Weight Nuclear Envelope / physiology* Nuclear Proteins Proteins / physiology* Rats |
| Grant Support | |
ID/Acronym/Agency:
|
AG07444/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 0/EEF1A2 protein, human; 0/Nuclear Proteins; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intraoperative fiberoptic angioscopy to evaluate the completeness of pulmonary embolectomy.
Next Document: Induction of polarized apical expression and vectorial release of carcinoembryonic antigen (CEA) dur...